Home LiteratureArticle Details
PMID: 15127437 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A droplet-based, composite PDMS/glass capillary microfluidic system for evaluating protein crystallization conditions by microbatch and vapor-diffusion methods with on-chip X-ray diffraction.

Angewandte Chemie (International ed. in English) ·Vol. 43 ·No. 19 ·2004-05-03 ·Pages 2508-11

Zheng B, Tice JD, Roach LS, Ismagilov RF

Abstract

暂无摘要

MeSH Terms
Crystallization/instrumentation,methods Crystallography, X-Ray/methods Diffusion Dimethylpolysiloxanes/chemistry Fluorocarbons/chemistry Glass/chemistry Microfluidics Oils/chemistry Plant Proteins/chemistry Proteins/chemistry Siloxanes/chemistry Sodium Chloride/chemistry Water/chemistry
Chemicals
Dimethylpolysiloxanes Fluorocarbons Oils Plant Proteins Proteins Siloxanes Water Sodium Chloride thaumatin protein, plant
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zheng Bo
MRSEC microfluidic facility, USA.
Tice Joshua D
Roach L Spencer
Ismagilov Rustem F
References (18)
18 references, click to expand
  1. The promise of macromolecular crystallization in microfluidic chips.
    J Struct Biol. 2003 Apr;142(1):180-7 PMID: 12718930
  2. Nitrogenase MoFe-protein at 1.16 A resolution: a central ligand in the FeMo-cofactor.
    Science. 2002 Sep 6;297(5587):1696-700 PMID: 12215645
  3. Nucleation of protein crystals in a wide continuous supersaturation gradient.
    Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1606-10 PMID: 12351871
  4. Real-space refinement of the structure of hen egg-white lysozyme.
    J Mol Biol. 1974 Jan 25;82(3):371-91 PMID: 4856347
  5. A microfluidic system for controlling reaction networks in time.
    Angew Chem Int Ed Engl. 2003 Feb 17;42(7):768-72 PMID: 12596195
  6. Nucleation of protein crystals.
    J Struct Biol. 2003 Apr;142(1):22-31 PMID: 12718916
  7. Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets.
    J Am Chem Soc. 2003 Sep 17;125(37):11170-1 PMID: 16220918
  8. Comparative studies of protein crystallization by vapour-diffusion and microbatch techniques.
    Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):8-15 PMID: 9761813
  9. Nanoflow solvent gradient delivery from a microfabricated device for protein identifications by electrospray ionization mass spectrometry.
    Anal Chem. 1998 Sep 15;70(18):3721-7 PMID: 9751016
  10. The prospects of protein nanocrystallography.
    Acta Crystallogr D Biol Crystallogr. 2002 Nov;58(Pt 11):1901-6 PMID: 12393920
  11. Microfluidic devices connected to fused-silica capillaries with minimal dead volume.
    Anal Chem. 1999 Aug 1;71(15):3292-6 PMID: 21662919
  12. A free interface diffusion technique for the crystallization of proteins for x-ray crystallography.
    Arch Biochem Biophys. 1972 Aug;151(2):533-9 PMID: 4625692
  13. Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme.
    Science. 2004 Jan 2;303(5654):76-9 PMID: 14704425
  14. High-throughput crystallography at an affordable cost: the TB Structural Genomics Consortium Crystallization Facility.
    Acc Chem Res. 2003 Mar;36(3):173-81 PMID: 12641474
  15. A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion.
    Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16531-6 PMID: 12486223
  16. Structures of three crystal forms of the sweet protein thaumatin.
    Acta Crystallogr D Biol Crystallogr. 1994 Nov 1;50(Pt 6):813-25 PMID: 15299348
  17. Investigations on protein crystal growth by the gel acupuncture method.
    Acta Crystallogr D Biol Crystallogr. 1994 Jul 1;50(Pt 4):484-90 PMID: 15299407
  18. A modified microdiffusion procedure for the growth of single protein crystals by concentration-gradient equilibrium dialysis.
    Arch Biochem Biophys. 1970 Dec;141(2):489-98 PMID: 5497144
Article Info
Journal
Angewandte Chemie (International ed. in English)
Abbr.
Angew Chem Int Ed Engl
ISSN
1433-7851
DOI
10.1002/anie.200453974
Published
2004-05-03
Pages
2508-11
Language
English
Region
Germany
NLM ID
0370543
PMCID
PMC1766324
Subset
IM
Grants
NCRR NIH HHS · P41 RR007707 · United States
NIBIB NIH HHS · R01 EB001903 · United States
NCRR NIH HHS · RR07707 · United States
External Links
PubMed source DOI: 10.1002/anie.200453974 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2