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PMID: 1508208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A cyclic AMP-responsive element-binding transcriptional activator in Drosophila melanogaster, dCREB-A, is a member of the leucine zipper family.

Molecular and cellular biology ·Vol. 12 ·No. 9 ·1992-09-00 ·Pages 4123-31

Smolik SM, Rose RE, Goodman RH

Abstract

In this report, we describe the isolation and initial characterization of a Drosophila protein, dCREB-A, that can bind the somatostatin cyclic AMP (cAMP)-responsive element and is capable of activating transcription in cell culture. Sequence analysis demonstrates that this protein is a member of the leucine zipper family of transcription factors. dCREB-A is unusual in that it contains six hydrophobic residue iterations in the zipper domain rather than the four or five commonly found in this group of proteins. The DNA-binding domain is more closely related to mammalian CREB than to the AP-1 factors in both sequence homology and specificity of cAMP-responsive element binding. In embryos, dCREB-A is expressed in the developing salivary gland. A more complex pattern of expression is detected in the adult; transcripts are found in the brain and optic lobe cell bodies, salivary gland, and midgut epithelial cells of the cardia. In females, dCREB-A is expressed in the ovarian columnar follicle cells, and in males, dCREB-A RNA is seen in the seminal vesicle, ejaculatory duct, and ejaculatory bulb. These results suggest that the dCREB-A transcription factor may be involved in fertility and neurological functions.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cyclic AMP Response Element-Binding Protein A DNA/metabolism DNA-Binding Proteins/genetics,isolation & purification,metabolism Drosophila Proteins Drosophila melanogaster/chemistry,genetics,growth & development Leucine Zippers Molecular Sequence Data Nucleic Acid Hybridization Sequence Alignment Trans-Activators/genetics,isolation & purification,metabolism Transcription Factors/genetics,isolation & purification,metabolism Transcription, Genetic
Chemicals
CrebA protein, Drosophila Cyclic AMP Response Element-Binding Protein A DNA-Binding Proteins Drosophila Proteins Trans-Activators Transcription Factors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smolik S M
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201-3098.
Rose R E
Goodman R H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.9.4123-4131.1992
Published
1992-09-00
Pages
4123-31
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360311
Subset
IM
Databases
GENBANK
M87038
External Links
PubMed source DOI: 10.1128/mcb.12.9.4123-4131.1992 PMC Full Text View journal details
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