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PMID: 2886975 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of the promoter sequences involved in the cell specific expression of the rat somatostatin gene.

Nucleic acids research ·Vol. 15 ·No. 14 ·1987-07-24 ·Pages 5715-28

Andrisani OM, Hayes TE, Roos B, Dixon JE

Abstract

DNA sequences containing the 5' flanking region of the rat somatostatin gene were linked to the coding sequence of the bacterial chloramphenicol acetyl transferase gene. This recombinant plasmid is active in expressing CAT activity in the neuronally derived, somatostatin producing CA-77 cell line. Deletion analyses of the somatostatin promoter show that the sequences proximal to position -60, relative to the cap site are required for expression of this promoter. A 4 base pair deletion of residues -46 through -43 within the somatostatin promoter results in a down mutation in vivo suggesting the existence of an element critical for the expression of the promoter in CA-77 cells. In addition, the somatostatin recombinant and its 5' deletion constructs preferentially express CAT activity in CA-77 cells, whereas only basal level of expression is observed in HeLa, BSC40, and RIN-5F cell lines, pointing to the cell specific nature of this promoter.

MeSH Terms
Acetyltransferases/genetics Base Sequence Cell Line Chloramphenicol O-Acetyltransferase DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial HeLa Cells/metabolism Humans Plasmids Promoter Regions, Genetic Somatostatin/genetics
Chemicals
Somatostatin Acetyltransferases Chloramphenicol O-Acetyltransferase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Andrisani O M
Hayes T E
Roos B
Dixon J E
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44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.14.5715
Published
1987-07-24
Pages
5715-28
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306017
Subset
IM
Grants
NIADDK NIH HHS · AM18024 · United States
NIADDK NIH HHS · AM36116 · United States
External Links
PubMed source DOI: 10.1093/nar/15.14.5715 PMC Full Text View journal details
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