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PMID: 15047853 Published · epublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExH/D box protein that is highly related to the mRNA export protein UAP56.

Nucleic acids research ·Vol. 32 ·No. 6 ·2004-00-00 ·Pages 1857-65

Pryor A, Tung L, Yang Z, Kapadia F, Chang TH, Johnson LF

Abstract

URH49 is a mammalian protein that is 90% identical to the DExH/D box protein UAP56, an RNA helicase that is important for splicing and nuclear export of mRNA. Although Saccharomyces cerevisiae and Drosophila express only a single protein corresponding to UAP56, mRNAs encoding URH49 and UAP56 are both expressed in human and mouse cells. Both proteins interact with the mRNA export factor Aly and both are able to rescue the loss of Sub2p (the yeast homolog of UAP56), indicating that both proteins have similar functions. UAP56 mRNA is more abundant than URH49 mRNA in many tissues, although in testes URH49 mRNA is much more abundant. UAP56 and URH49 mRNAs are present at similar levels in proliferating cultured cells. However, when the cells enter quiescence, the URH49 mRNA level decreases 3-6-fold while the UAP56 mRNA level remains relatively constant. The amount of URH49 mRNA increases to the level found in proliferating cells within 5 h when quiescent cells are growth-stimulated or when protein synthesis is inhibited. URH49 mRNA is relatively unstable (T(1/2) = 4 h) in quiescent cells, but is stabilized immediately following growth stimulation or inhibition of protein synthesis. In contrast, there is much less change in the content or stability of UAP56 mRNA following growth stimulation. Our observations suggest that in mammalian cells, two UAP56-like RNA helicases are involved in splicing and nuclear export of mRNA. Differential expression of these helicases may lead to quantitative or qualitative changes in mRNA expression.

MeSH Terms
ATP-Binding Cassette Transporters Active Transport, Cell Nucleus Adenosine Triphosphatases/genetics Animals Cell Division Cell Nucleus/metabolism Conserved Sequence DEAD-box RNA Helicases Gene Expression Regulation Genetic Complementation Test HeLa Cells Humans Male Mice Nuclear Proteins/metabolism RNA Helicases/genetics,metabolism,physiology RNA Stability RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Resting Phase, Cell Cycle Transcription Factors/metabolism
Chemicals
ALYREF protein, human ATP-Binding Cassette Transporters Nuclear Proteins RNA, Messenger RNA-Binding Proteins Refbp1 protein, mouse Transcription Factors Adenosine Triphosphatases DDX39A protein, human DDX39B protein, human RNA-dependent ATPase DEAD-box RNA Helicases RNA Helicases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pryor Anne
The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA.
Tung Luh
Yang Zhe
Kapadia Fehmida
Chang Tien-Hsien
Johnson Lee F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkh347
Published
2004-00-00
Epub
2004-00-26
Pages
1857-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC390356
Subset
IM
Grants
NCI NIH HHS · P30 CA016058 · United States
NCI NIH HHS · CA16058 · United States
NIGMS NIH HHS · GM29356 · United States
NIGMS NIH HHS · GM48752 · United States
External Links
PubMed source DOI: 10.1093/nar/gkh347 PMC Full Text View journal details
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