Home LiteratureArticle Details
PMID: 15044704 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The spatial orientation of Helicobacter pylori in the gastric mucus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 14 ·2004-04-06 ·Pages 5024-9

Schreiber S, Konradt M, Groll C, Scheid P, Hanauer G, Werling HO, Josenhans C, Suerbaum S

Abstract

The highly motile human pathogen Helicobacter pylori lives deep in the gastric mucus layer. To identify which chemical gradient guides the bacteria within the mucus layer, combinations of luminal perfusion, dialysis, and ventilation were used to modify or invert transmucus gradients in anaesthetized Helicobacter-infected mice and Mongolian gerbils. Neither changes in lumen or arterial pH nor inversion of bicarbonate/CO2 or urea/ammonium gradients disturbed Helicobacter orientation. However, elimination of the mucus pH gradient by simultaneous reduction of arterial pH and bicarbonate concentration perturbed orientation, causing the bacteria to spread over the entire mucus layer. H. pylori thus uses the gastric mucus pH gradient for chemotactic orientation.

MeSH Terms
Animals Bicarbonates/metabolism Carbon Dioxide/metabolism Female Gastric Mucosa/microbiology Helicobacter pylori/metabolism,physiology Hydrogen-Ion Concentration Mice Quaternary Ammonium Compounds/metabolism Urea/metabolism
Chemicals
Bicarbonates Quaternary Ammonium Compounds Carbon Dioxide Urea
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Schreiber Sören
Institut für Physiologie, Ruhr-Universität Bochum, D-44780 Bochum, Germany. soeren.shreiber@ruhr-uni-bochum.de
Konradt Manuela
Groll Claudia
Scheid Peter
Hanauer Guido
Werling Hans-Otto
Josenhans Christine
Suerbaum Sebastian
References (36)
36 references, click to expand
  1. Cytoplasmic pH mediates pH taxis and weak-acid repellent taxis of bacteria.
    J Bacteriol. 1981 Mar;145(3):1209-21 PMID: 7009572
  2. Gastric bicarbonate secretion: an update.
    Brain Res Bull. 1980;5 Suppl 1:15-8 PMID: 6992944
  3. A demonstration of bicarbonate production by the normal human stomach in vivo.
    Dig Dis Sci. 1982 Nov;27(11):961-6 PMID: 7140493
  4. Gastroduodenal defence mechanisms.
    Clin Gastroenterol. 1984 May;13(2):327-54 PMID: 6378442
  5. Intracellular pH (pHi) in gastric surface epithelium is more susceptible to serosal than mucosal acidification.
    Surgery. 1987 Aug;102(2):371-9 PMID: 3039678
  6. The gastric juice urea and ammonia levels in patients with Campylobacter pylori.
    Am J Clin Pathol. 1990 Aug;94(2):187-91 PMID: 2371972
  7. Effects of H+ and HCO3- secretion on mucus gel pH in isolated antral mucosa of bullfrog stomach.
    Bull Osaka Med Coll. 1990 Nov;36(1-2):13-25 PMID: 2135641
  8. Stability of pH gradients in vivo across the stomach in Helicobacter pylori gastritis, dyspepsia, and health.
    Am J Gastroenterol. 1992 May;87(5):590-4 PMID: 1595645
  9. The importance of local acid production in the distribution of Helicobacter felis in the mouse stomach.
    Gastroenterology. 1995 May;108(5):1386-95 PMID: 7729630
  10. The dynamics of Helicobacter pylori infection of the human stomach.
    J Theor Biol. 1995 Sep 21;176(2):281-90 PMID: 7475116
  11. Role of the host in pathogenesis of Helicobacter-associated gastritis: H. felis infection of inbred and congenic mouse strains.
    Infect Immun. 1996 Jan;64(1):238-45 PMID: 8557346
  12. Gastric mucus of the guinea pig: proton carrier and diffusion barrier.
    Am J Physiol. 1997 Jan;272(1 Pt 1):G63-70 PMID: 9038877
  13. Urease-independent chemotactic responses of Helicobacter pylori to urea, urease inhibitors, and sodium bicarbonate.
    Infect Immun. 1997 Apr;65(4):1519-21 PMID: 9119496
  14. A standardized mouse model of Helicobacter pylori infection: introducing the Sydney strain.
    Gastroenterology. 1997 Apr;112(4):1386-97 PMID: 9098027
  15. Epithelial attachment alters the outcome of Helicobacter pylori infection.
    Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3925-30 PMID: 9520469
  16. Urease plays an important role in the chemotactic motility of Helicobacter pylori in a viscous environment.
    Infect Immun. 1998 Oct;66(10):4832-7 PMID: 9746586
  17. Free recombination within Helicobacter pylori.
    Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12619-24 PMID: 9770535
  18. Micropipette system for sampling and injecting small volumes.
    J Neurosci Methods. 1998 Nov 1;85(1):27-32 PMID: 9874138
  19. Helicobacter pylori-induced chronic active gastritis, intestinal metaplasia, and gastric ulcer in Mongolian gerbils.
    Am J Pathol. 1999 Mar;154(3):951-60 PMID: 10079274
  20. PAS domains: internal sensors of oxygen, redox potential, and light.
    Microbiol Mol Biol Rev. 1999 Jun;63(2):479-506 PMID: 10357859
  21. In vivo distribution of Helicobacter felis in the gastric mucus of the mouse: experimental method and results.
    Infect Immun. 1999 Oct;67(10):5151-6 PMID: 10496889
  22. The relationship between Helicobacter pylori motility, morphology and phase of growth: implications for gastric colonization and pathology.
    Microbiology. 1999 Oct;145 ( Pt 10):2803-11 PMID: 10537202
  23. Aerotaxis and other energy-sensing behavior in bacteria.
    Annu Rev Microbiol. 1999;53:103-28 PMID: 10547687
  24. PAS domain residues involved in signal transduction by the Aer redox sensor of Escherichia coli.
    Mol Microbiol. 2000 May;36(4):806-16 PMID: 10844669
  25. Demonstration of a pH gradient in the gastric gland of the acid-secreting guinea pig mucosa.
    Am J Physiol Gastrointest Liver Physiol. 2000 Sep;279(3):G597-604 PMID: 10960360
  26. Energy taxis is the dominant behavior in Azospirillum brasilense.
    J Bacteriol. 2000 Nov;182(21):6042-8 PMID: 11029423
  27. A novel action of the proton pump inhibitor rabeprazole and its thioether derivative against the motility of Helicobacter pylori.
    Antimicrob Agents Chemother. 2000 Nov;44(11):3069-73 PMID: 11036024
  28. How signals are heard during bacterial chemotaxis: protein-protein interactions in sensory signal propagation.
    J Bacteriol. 2000 Dec;182(24):6865-73 PMID: 11092844
  29. A transgenic model for listeriosis: role of internalin in crossing the intestinal barrier.
    Science. 2001 Jun 1;292(5522):1722-5 PMID: 11387478
  30. Globin-coupled sensors: a class of heme-containing sensors in Archaea and Bacteria.
    Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9353-8 PMID: 11481493
  31. Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age.
    Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15056-61 PMID: 11742075
  32. Helicobacter pylori infection.
    N Engl J Med. 2002 Oct 10;347(15):1175-86 PMID: 12374879
  33. The impact of parietal cells on Helicobacter pylori tropism and host pathology: an analysis using gnotobiotic normal and transgenic mice.
    Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3467-72 PMID: 12629225
  34. pH-regulated gene expression of the gastric pathogen Helicobacter pylori.
    Infect Immun. 2003 Jun;71(6):3529-39 PMID: 12761138
  35. Carbon dioxide tensions in the proximal part of the canine gastrointestinal tract.
    Gastroenterology. 1969 Apr;56(4):758-62 PMID: 5821083
  36. High plasma HCO3-protects gastric mucosa against acute ulceration in the rat.
    Gastroenterology. 1981 Nov;81(5):921-7 PMID: 6269947
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0308386101
Email
soeren.shreiber@ruhr-uni-bochum.de
Published
2004-04-06
Epub
2004-00-25
Pages
5024-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387367
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0308386101 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2