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PMID: 15024081 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3.

Molecular and cellular biology ·Vol. 24 ·No. 7 ·2004-04-00 ·Pages 2932-43

Cheng H, He X, Moore C

Abstract

Swd2, an essential WD repeat protein in Saccharomyces cerevisiae, is a component of two very different complexes: the cleavage and polyadenylation factor CPF and the Set1 methylase, which modifies lysine 4 of histone H3 (H3-K4). It was not known if Swd2 is important for the function of either of these entities. We show here that, in extract from cells depleted of Swd2, cleavage and polyadenylation of the mRNA precursor in vitro are completely normal. However, temperature-sensitive mutations or depletion of Swd2 causes termination defects in some genes transcribed by RNA polymerase II. Overexpression of Ref2, a protein previously implicated in snoRNA 3' end formation and Swd2 recruitment to CPF, can rescue the growth and termination defects, indicating a functional interaction between the two proteins. Some swd2 mutations also significantly decrease global H3-K4 methylation and cause other phenotypes associated with loss of this chromatin modification, such as loss of telomere silencing, hydroxyurea sensitivity, and alterations in repression of INO1 transcription. Even though the two Swd2-containing complexes are both localized to actively transcribed genes, the allele specificities of swd2 defects suggest that the functions of Swd2 in mediating RNA polymerase II termination and H3-K4 methylation are not tightly coupled.

MeSH Terms
Histone-Lysine N-Methyltransferase Histones/metabolism Lysine/metabolism Methylation Phenotype RNA Polymerase II/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins/genetics,metabolism Temperature Transcription, Genetic
Chemicals
Histones REF2 protein, S cerevisiae RNA, Messenger RNA-Binding Proteins Saccharomyces cerevisiae Proteins Histone-Lysine N-Methyltransferase SWD2 protein, S cerevisiae RNA Polymerase II Lysine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cheng Hailing
Tufts University School of Medicine and Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts 02111, USA.
He Xiaoyuan
Moore Claire
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.7.2932-2943.2004
Published
2004-04-00
Pages
2932-43
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC371121
Subset
IM
Grants
NIGMS NIH HHS · R01 GM041752 · United States
NIGMS NIH HHS · R01 GM068887 · United States
NIGMS NIH HHS · GM041752 · United States
NIGMS NIH HHS · GM068887 · United States
External Links
PubMed source DOI: 10.1128/MCB.24.7.2932-2943.2004 PMC Full Text View journal details
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