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PMID: 15004005 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Role of the processing pore of the ClpX AAA+ ATPase in the recognition and engagement of specific protein substrates.

Genes & development ·Vol. 18 ·No. 4 ·2004-02-15 ·Pages 369-74

Siddiqui SM, Sauer RT, Baker TA

Abstract

ClpX binds substrates bearing specific classes of peptide signals, denatures these proteins, and translocates them through a central pore into ClpP for degradation. ClpX with the V154F po e mutation is severely defective in binding substrates bearing C-motif 1 degradation signals and is also impaired in a subsequent step of substrate engagement. In contrast, this mutant efficiently processes substrates with other classes of recognition signals both in vitro and in vivo. These results demonstrate that the ClpX pore functions in the recognition and catalytic engagement of specific substrates, and that ClpX recognizes different substrate classes in at least two distinct fashions.

MeSH Terms
ATPases Associated with Diverse Cellular Activities Adenosine Triphosphatases/chemistry,genetics,metabolism Amino Acid Sequence Binding Sites Catalysis Endopeptidase Clp Escherichia coli Proteins Kinetics Molecular Chaperones Molecular Sequence Data Mutagenesis Peptide Fragments/chemistry Protein Transport Recombinant Proteins/chemistry,metabolism Restriction Mapping Substrate Specificity
Chemicals
Escherichia coli Proteins Molecular Chaperones Peptide Fragments Recombinant Proteins Endopeptidase Clp Adenosine Triphosphatases ClpX protein, E coli ATPases Associated with Diverse Cellular Activities
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Siddiqui Samia M
Massachusetts Institute of Technology, Department of Biology, Howard Hughes Medical Institute, Cambridge, Massachusetts 02139, USA.
Sauer Robert T
Baker Tania A
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1170304
Published
2004-02-15
Pages
369-74
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC359390
Subset
IM
Grants
NIAID NIH HHS · R01 AI016892 · United States
NIGMS NIH HHS · R01 GM049224 · United States
NIGMS NIH HHS · R01 GM049224-11 · United States
NIAID NIH HHS · AI-16892 · United States
External Links
PubMed source DOI: 10.1101/gad.1170304 PMC Full Text View journal details
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