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PMID: 14970327 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inactivation of a Pseudomonas aeruginosa quorum-sensing signal by human airway epithelia.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 10 ·2004-03-09 ·Pages 3587-90

Chun CK, Ozer EA, Welsh MJ, Zabner J, Greenberg EP

Abstract

Mammalian airways protect themselves from bacterial infection by using multiple defense mechanisms including antimicrobial peptides, mucociliary clearance, and phagocytic cells. We asked whether airways might also target a key bacterial cell-cell communication system, quorum-sensing. The opportunistic pathogen Pseudomonas aeruginosa uses two quorum-sensing molecules, N-(3-oxododecanoyl)-l-homoserine lactone (3OC12-HSL) and N-butanoyl-l-homoserine lactone (C4-HSL), to control production of extracellular virulence factors and biofilm formation. We found that differentiated human airway epithelia inactivated 3OC12-HSL. Inactivation was selective for acyl-HSLs with certain acyl side chains, and C4-HSL was not inactivated. In addition, the capacity for inactivation varied widely in different cell types. 3OC12-HSL was inactivated by a cell-associated activity rather than a secreted factor. These data suggest that the ability of human airway epithelia to inactivate quorum-sensing signal molecules could play a role in the innate defense against bacterial infection.

MeSH Terms
4-Butyrolactone/analogs & derivatives,antagonists & inhibitors,physiology Animals CHO Cells COS Cells Cell Line Cell Membrane/physiology Cells, Cultured Cricetinae Dogs HeLa Cells Homoserine/analogs & derivatives,antagonists & inhibitors,physiology Humans Immunity, Innate Pseudomonas aeruginosa/immunology,pathogenicity,physiology Respiratory Mucosa/immunology,microbiology Signal Transduction Virulence/physiology
Chemicals
N-(3-oxododecanoyl)homoserine lactone N-butyrylhomoserine lactone Homoserine 4-Butyrolactone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chun Carlene K
Pacific Biomedical Research Center, Kewalo Marine Laboratory, 41 Ahui Street, University of Hawaii, Honolulu, HI 96813, USA.
Ozer Egon A
Welsh Michael J
Zabner Joseph
Greenberg E P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0308750101
Published
2004-03-09
Epub
2004-00-17
Pages
3587-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC373506
Subset
IM
Grants
NHLBI NIH HHS · P01 HL051670 · United States
NHLBI NIH HHS · HL61234 · United States
NIDDK NIH HHS · DK54759 · United States
NIGMS NIH HHS · R01 GM059026 · United States
NHLBI NIH HHS · HL51670 · United States
NHLBI NIH HHS · P50 HL061234 · United States
NIGMS NIH HHS · GM59026 · United States
NIDDK NIH HHS · P30 DK054759 · United States
NIGMS NIH HHS · T32 GM007337 · United States
Corrections
CommentIn
PMID: 15024108
External Links
PubMed source DOI: 10.1073/pnas.0308750101 PMC Full Text View journal details
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