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PMID: 1496003 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

dOct2, a Drosophila Oct transcription factor that functions in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 7080-4

Prakash K, Fang XD, Engelberg D, Behal A, Parker CS

Abstract

Oct factors are members of the POU family of transcription factors that are shown to play important roles during development in mammals. Here we report the cDNA cloning and expression of a Drosophila Oct transcription factor. Whole mount in situ hybridization experiments revealed that the spatial expression patterns of this gene during embryonic development have not yet been observed for any other gene. In early embryogenesis, its transcripts are transiently expressed as a wide uniform band from 20% to 40% of the egg length, very similar to that of gap genes. This pattern progressively resolves into a series of narrower stripes followed by expression in 14 stripes. Subsequently, transcripts from this gene are expressed in the central nervous system and the brain. When expressed in the yeast Saccharomyces cerevisiae, this Drosophila factor functions as a strong, octamer-dependent activator of transcription. Our data strongly suggest possible functions for the Oct factor in pattern formation in Drosophila that might transcend the boundaries of genetically defined segmentation genes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics DNA-Binding Proteins/metabolism Drosophila/genetics,metabolism Embryo, Nonmammalian Gene Library Humans Molecular Sequence Data Octamer Transcription Factor-2 Plasmids Protein Conformation Recombinant Fusion Proteins/isolation & purification,metabolism Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Transcription Factors/genetics,isolation & purification,metabolism beta-Galactosidase/genetics,isolation & purification,metabolism
Chemicals
DNA-Binding Proteins Octamer Transcription Factor-2 POU2F2 protein, human Recombinant Fusion Proteins Transcription Factors DNA beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Prakash K
Division of Chemistry, California Institute of Technology, Pasadena 91125.
Fang X D
Engelberg D
Behal A
Parker C S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.7080
Published
1992-08-01
Pages
7080-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49649
Subset
IM
Grants
NIGMS NIH HHS · GM42671 · United States
Databases
GENBANK
M93149
External Links
PubMed source DOI: 10.1073/pnas.89.15.7080 PMC Full Text View journal details
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