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PMID: 14749381 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mouse strains with an active H2-Ea meiotic recombination hot spot exhibit increased levels of H2-Ea-specific DNA breaks in testicular germ cells.

Molecular and cellular biology ·Vol. 24 ·No. 4 ·2004-02-00 ·Pages 1655-66

Qin J, Richardson LL, Jasin M, Handel MA, Arnheim N

Abstract

We devised a sensitive method for the site-specific detection of rare meiotic DNA strand breaks in germ cell-enriched testicular cell populations from mice that possess or lack an active recombination hot spot at the H2-Ea gene. Using germ cells from adult animals, we found an excellent correlation between the frequency of DNA breaks in the 418-bp H2-Ea hot spot and crossover activity. The temporal appearance of DNA breaks was also studied in 7- to 18-day-old mice with an active hot spot during the first waves of spermatogenesis. The number of DNA breaks detected rose as leptotene and zygotene spermatocytes populate the testis with a peak at day 14 postpartum, when leptotene, zygotene, and early pachytene spermatocytes are the most common meiotic prophase I cell types. The number of DNA breaks drops precipitously 1 day later, when middle to late pachytene spermatocytes become the dominant subtype. The recombination-related breaks in the hot spot likely reflect SPO11-induced double-strand breaks and/or recombination intermediates containing free 3' hydroxyl groups.

MeSH Terms
Animals Base Sequence DNA Damage/genetics Female Germ Cells/metabolism H-2 Antigens/genetics Haplotypes/genetics Heterozygote Male Meiosis/genetics Mice Molecular Sequence Data Recombination, Genetic/genetics Testis/cytology,metabolism
Chemicals
H-2 Antigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Qin Jian
Program in Molecular and Computational Biology, University of Southern California, Los Angeles, California 90089-1340, USA.
Richardson Laura L
Jasin Maria
Handel Mary Ann
Arnheim Norman
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.4.1655-1666.2004
Published
2004-02-00
Pages
1655-66
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC344187
Subset
IM
Grants
NICHD NIH HHS · HD33816 · United States
NIGMS NIH HHS · R37 GM036745 · United States
NIGMS NIH HHS · GM36745 · United States
NICHD NIH HHS · R01 HD033816 · United States
NICHD NIH HHS · HD31376 · United States
NIGMS NIH HHS · R01 GM036745 · United States
External Links
PubMed source DOI: 10.1128/MCB.24.4.1655-1666.2004 PMC Full Text View journal details
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