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PMID: 14715903 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence for polar positional information independent of cell division and nucleoid occlusion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 3 ·2004-01-20 ·Pages 835-40

Janakiraman A, Goldberg MB

Abstract

We present evidence that, in Escherichia coli, polar positional information is present at midcell independent of known cell division factors. In filamented cells, IcsA, which is normally polar, localizes at or near potential cell division sites. Because the cell pole is derived from the septum, the sites to which IcsA localizes in filaments correspond to future poles. IcsA localization to these sites is independent of FtsZ, MinCDE, septation, and nucleoid occlusion, indicating that positional information for the future pole is independent of cell division and chromosome positioning. Upon IcsA localization to these sites, septation is inhibited, suggesting that IcsA recognition of this polar positional information may influence cell division.

MeSH Terms
Bacterial Proteins Cell Division Cell Nucleus/metabolism,ultrastructure Cell Polarity DNA-Binding Proteins/genetics,metabolism Escherichia coli/genetics,metabolism,ultrastructure Escherichia coli Proteins/genetics,metabolism Green Fluorescent Proteins Luminescent Proteins/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Subcellular Fractions/metabolism Transcription Factors/genetics,metabolism
Chemicals
Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins FtsZ84 protein, E coli Luminescent Proteins Recombinant Fusion Proteins Transcription Factors virG protein, Shigella flexneri Green Fluorescent Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Janakiraman Anuradha
Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital/Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.
Goldberg Marcia B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0305747101
Published
2004-01-20
Epub
2004-00-08
Pages
835-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC321767
Subset
IM
Grants
NIAID NIH HHS · R01 AI035817 · United States
NIAID NIH HHS · AI35817 · United States
External Links
PubMed source DOI: 10.1073/pnas.0305747101 PMC Full Text View journal details
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