Home LiteratureArticle Details
PMID: 8468279 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement.

Journal of bacteriology ·Vol. 175 ·No. 8 ·1993-04-00 ·Pages 2189-96

Goldberg MB, Bârzu O, Parsot C, Sansonetti PJ

Abstract

Shigella flexneri uses elements of the host cell cytoskeleton to move within cells and from cell to cell. IcsA, an S. flexneri protein involved in this movement, was purified and studied in vitro. IcsA bound the radiolabelled ATP analog 3'(2')-O-(4-benzoyl)benzoyl-ATP and hydrolyzed ATP. In addition, the surface localization of IcsA on both extracellular and intracellular shigellae was unipolar. Further, in HeLa cells infected with shigellae, IcsA antiserum labelled the actin tail throughout its length, thereby suggesting that IcsA interacts with elements within the tail. Localization of IcsA within the tail at a distance from the bacterium would require its secretion; we demonstrate here that in vitro IcsA is secreted into the culture supernatant in a cleaved form.

MeSH Terms
Adenosine Triphosphatases/analysis Adenosine Triphosphate/metabolism Amino Acid Sequence Bacterial Proteins/analysis,chemistry,isolation & purification,metabolism Cell Movement DNA-Binding Proteins Escherichia coli/metabolism HeLa Cells Humans Molecular Sequence Data Shigella flexneri/chemistry Transcription Factors
Chemicals
Bacterial Proteins DNA-Binding Proteins Transcription Factors virG protein, Shigella flexneri Adenosine Triphosphate Adenosine Triphosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Goldberg M B
Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, France.
Bârzu O
Parsot C
Sansonetti P J
References (49)
49 references, click to expand
  1. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.
    Biochem J. 1964 Jan;90(1):147-9 PMID: 5832284
  2. Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6068-72 PMID: 2117270
  3. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  4. Fimbrin is a cytoskeletal protein that crosslinks F-actin in vitro.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6849-53 PMID: 6947259
  5. Involvement of a plasmid in the invasive ability of Shigella flexneri.
    Infect Immun. 1982 Mar;35(3):852-60 PMID: 6279518
  6. Fimbrin is a homologue of the cytoplasmic phosphoprotein plastin and has domains homologous with calmodulin and actin gelation proteins.
    J Cell Biol. 1990 Sep;111(3):1069-79 PMID: 2391360
  7. Actin filament nucleation by the bacterial pathogen, Listeria monocytogenes.
    J Cell Biol. 1990 Dec;111(6 Pt 2):2979-88 PMID: 2125302
  8. Two novel virulence loci, mxiA and mxiB, in Shigella flexneri 2a facilitate excretion of invasion plasmid antigens.
    Infect Immun. 1991 Jun;59(6):1997-2005 PMID: 2037361
  9. The ferric-pseudobactin receptor PupA of Pseudomonas putida WCS358: homology to TonB-dependent Escherichia coli receptors and specificity of the protein.
    Mol Microbiol. 1991 Mar;5(3):647-55 PMID: 1646376
  10. Conservation of components of the Escherichia coli export machinery in prokaryotes.
    FEMS Microbiol Lett. 1991 May 15;64(2-3):195-9 PMID: 1679403
  11. Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin.
    Infect Immun. 1991 Oct;59(10):3463-71 PMID: 1910001
  12. Myosin subfragment-1 interacts with two G-actin molecules in the absence of ATP.
    J Biol Chem. 1991 Sep 25;266(27):17872-9 PMID: 1917928
  13. FtsZ ring structure associated with division in Escherichia coli.
    Nature. 1991 Nov 14;354(6349):161-4 PMID: 1944597
  14. Isolation and serologic characterization of AIDA-I, the adhesin mediating the diffuse adherence phenotype of the diarrhea-associated Escherichia coli strain 2787 (O126:H27).
    Infect Immun. 1992 Jan;60(1):13-8 PMID: 1729177
  15. L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein.
    Cell. 1992 Feb 7;68(3):521-31 PMID: 1739966
  16. Movement along actin filaments of the perijunctional area and de novo polymerization of cellular actin are required for Shigella flexneri colonization of epithelial Caco-2 cell monolayers.
    Infect Immun. 1992 Mar;60(3):1031-40 PMID: 1541518
  17. Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus.
    J Bacteriol. 1992 Mar;174(6):1990-2001 PMID: 1312536
  18. Identification and characterization of B-cell epitopes of IpaC, an invasion-associated protein of Shigella flexneri.
    Infect Immun. 1992 May;60(5):1919-26 PMID: 1373401
  19. Polar localization of a bacterial chemoreceptor.
    Genes Dev. 1992 May;6(5):825-36 PMID: 1577276
  20. The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization.
    Nature. 1992 May 21;357(6375):257-60 PMID: 1589024
  21. Phosphorylation of IcsA by cAMP-dependent protein kinase and its effect on intracellular spread of Shigella flexneri.
    Mol Microbiol. 1992 Apr;6(7):833-41 PMID: 1602963
  22. How Listeria exploits host cell actin to form its own cytoskeleton. II. Nucleation, actin filament polarity, filament assembly, and evidence for a pointed end capper.
    J Cell Biol. 1992 Jul;118(1):83-93 PMID: 1618909
  23. AIDA-I, the adhesin involved in diffuse adherence of the diarrhoeagenic Escherichia coli strain 2787 (O126:H27), is synthesized via a precursor molecule.
    Mol Microbiol. 1992 Jun;6(11):1539-46 PMID: 1625582
  24. icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread.
    Mol Microbiol. 1992 Jun;6(12):1605-16 PMID: 1495389
  25. Host cell actin assembly is necessary and likely to provide the propulsive force for intracellular movement of Listeria monocytogenes.
    Infect Immun. 1992 Sep;60(9):3609-19 PMID: 1500169
  26. Unipolar reorganization of F-actin layer at bacterial division and bundling of actin filaments by plastin correlate with movement of Shigella flexneri within HeLa cells.
    Infect Immun. 1992 Oct;60(10):4088-99 PMID: 1398922
  27. MxiD, an outer membrane protein necessary for the secretion of the Shigella flexneri lpa invasins.
    Mol Microbiol. 1993 Jan;7(1):59-68 PMID: 8437520
  28. OmpB (osmo-regulation) and icsA (cell-to-cell spread) mutants of Shigella flexneri: vaccine candidates and probes to study the pathogenesis of shigellosis.
    Vaccine. 1991 Jun;9(6):416-22 PMID: 1887672
  29. Genetic analysis of protein export in Escherichia coli.
    Annu Rev Genet. 1990;24:215-48 PMID: 2088168
  30. Stress fiber-based movement of Shigella flexneri within cells.
    Infect Immun. 1991 May;59(5):1723-32 PMID: 2019438
  31. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides.
    J Mol Biol. 1983 Jun 25;167(2):391-409 PMID: 6345794
  32. A monoclonal antibody against a 135-K Golgi membrane protein.
    EMBO J. 1982;1(12):1621-8 PMID: 7188254
  33. DNA sequence of the F traALE region that includes the gene for F pilin.
    J Bacteriol. 1984 Oct;160(1):395-401 PMID: 6090426
  34. The 110,000-dalton actin- and calmodulin-binding protein from intestinal brush border is a myosin-like ATPase.
    J Biol Chem. 1984 Nov 25;259(22):14128-35 PMID: 6094541
  35. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  36. Nucleotide sequence and expression of a phosphate-regulated gene encoding a secreted hemolysin of Pseudomonas aeruginosa.
    J Bacteriol. 1986 Jul;167(1):291-8 PMID: 3087958
  37. A genetic determinant required for continuous reinfection of adjacent cells on large plasmid in S. flexneri 2a.
    Cell. 1986 Aug 15;46(4):551-5 PMID: 3524856
  38. Bacteriophage lambda cloning system for the construction of directional cDNA libraries.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4171-5 PMID: 2438693
  39. The 110-kD protein-calmodulin complex of the intestinal microvillus is an actin-activated MgATPase.
    J Cell Biol. 1987 Jul;105(1):313-24 PMID: 2956266
  40. ATPase activity of the microvillar 110 kDa polypeptide-calmodulin complex is activated in Mg2+ and inhibited in K+-EDTA by F-actin.
    FEBS Lett. 1987 Dec 10;225(1-2):269-72 PMID: 2961614
  41. Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2568-72 PMID: 3357883
  42. Intracellular spread of Shigella flexneri associated with the kcpA locus and a 140-kilodalton protein.
    Infect Immun. 1989 Feb;57(2):477-86 PMID: 2643571
  43. virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence.
    J Bacteriol. 1989 Jan;171(1):353-9 PMID: 2644195
  44. Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin.
    Proc Natl Acad Sci U S A. 1989 May;86(10):3867-71 PMID: 2542950
  45. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes.
    J Cell Biol. 1989 Oct;109(4 Pt 1):1597-608 PMID: 2507553
  46. Peptidoglycan synthesis during the cell cycle of Escherichia coli: composition and mode of insertion.
    J Bacteriol. 1989 Nov;171(11):5783-94 PMID: 2681142
  47. Intracellular and cell-to-cell spread of Listeria monocytogenes involves interaction with F-actin in the enterocytelike cell line Caco-2.
    Infect Immun. 1990 Apr;58(4):1048-58 PMID: 2108086
  48. The signal peptide.
    J Membr Biol. 1990 May;115(3):195-201 PMID: 2197415
  49. Cinemicrographic study of tissue cell cultures infected with Shigella flexneri.
    Jpn J Med Sci Biol. 1968 Aug;21(4):259-73 PMID: 4884612
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.8.2189-2196.1993
Published
1993-04-00
Pages
2189-96
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204503
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.175.8.2189-2196.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2