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PMID: 14688291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Programmed cell death remodels lace plant leaf shape during development.

The Plant cell ·Vol. 16 ·No. 1 ·2004-01-00 ·Pages 60-73

Gunawardena AH, Greenwood JS, Dengler NG

Abstract

Programmed cell death (PCD) functions in the developmental remodeling of leaf shape in higher plants, a process analogous to digit formation in the vertebrate limb. In this study, we provide a cytological characterization of the time course of events as PCD remodels young expanding leaves of the lace plant. Tonoplast rupture is the first PCD event in this system, indicated by alterations in cytoplasmic streaming, loss of anthocyanin color, and ultrastructural appearance. Nuclei become terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling positive soon afterward but do not become morphologically altered until late stages of PCD. Genomic DNA is fragmented, but not into internucleosomal units. Other cytoplasmic changes, such as shrinkage and degradation of organelles, occur later. This form of PCD resembles tracheary element differentiation in cytological execution but requires unique developmental regulation so that discrete panels of tissue located equidistantly between veins undergo PCD while surrounding cells do not.

MeSH Terms
Anthocyanins/metabolism Apoptosis Cell Differentiation Cell Nucleus/metabolism Cell Wall/metabolism Cytoplasmic Streaming/genetics,physiology DNA Fragmentation Magnoliopsida/genetics,growth & development,ultrastructure Microscopy, Electron Plant Leaves/genetics,growth & development,ultrastructure Vacuoles/metabolism
Chemicals
Anthocyanins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gunawardena Arunika H L A N
Department of Botany, University of Toronto, Toronto, Ontario, Canada M5S 1A1.
Greenwood John S
Dengler Nancy G
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.016188
Published
2004-01-00
Epub
2003-00-19
Pages
60-73
Language
English
Region
England
NLM ID
9208688
PMCID
PMC301395
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.016188 PMC Full Text View journal details
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