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PMID: 14673161 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mouse Murr1 gene is imprinted in the adult brain, presumably due to transcriptional interference by the antisense-oriented U2af1-rs1 gene.

Molecular and cellular biology ·Vol. 24 ·No. 1 ·2004-01-00 ·Pages 270-9

Wang Y, Joh K, Masuko S, Yatsuki H, Soejima H, Nabetani A, Beechey CV, Okinami S, Mukai T

Abstract

The mouse Murr1 gene contains an imprinted gene, U2af1-rs1, in its first intron. U2af1-rs1 shows paternal allele-specific expression and is transcribed in the direction opposite to that of the Murr1 gene. In contrast to a previous report of biallelic expression of Murr1 in neonatal mice, we have found that the maternal allele is expressed predominantly in the adult brain and also preferentially in other adult tissues. This maternal-predominant expression is not observed in embryonic and neonatal brains. In situ hybridization experiments that used the adult brain indicated that Murr1 gene was maternally expressed in neuronal cells in all regions of the brain. We analyzed the developmental change in the expression levels of both Murr1 and U2af1-rs1 in the brain and liver, and we propose that the maternal-predominant expression of Murr1 results from transcriptional interference of the gene by U2af1-rs1 through the Murr1 promoter region.

MeSH Terms
Adaptor Proteins, Signal Transducing Animals Brain/metabolism Genomic Imprinting In Situ Hybridization Introns Mice Nerve Tissue Proteins/genetics,metabolism Nuclear Proteins Promoter Regions, Genetic Proteins/genetics,metabolism Ribonucleoproteins Splicing Factor U2AF
Chemicals
Adaptor Proteins, Signal Transducing Commd1 protein, mouse Nerve Tissue Proteins Nuclear Proteins Proteins Ribonucleoproteins Splicing Factor U2AF Zrsr1 protein, mouse
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Wang Youdong
Department of Biomolecular Sciences, Saga Medical School, Saga 849-8501, Japan.
Joh Keiichiro
Masuko Sadahiko
Yatsuki Hitomi
Soejima Hidenobu
Nabetani Akira
Beechey Colin V
Okinami Satoshi
Mukai Tsunehiro
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.24.1.270-279.2004
Published
2004-01-00
Pages
270-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC303337
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.24.1.270-279.2004 PMC Full Text View journal details
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