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PMID: 8284201 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new imprinted gene cloned by a methylation-sensitive genome scanning method.

Nucleic acids research ·Vol. 21 ·No. 24 ·1993-12-11 ·Pages 5577-82

Hatada I, Sugama T, Mukai T

Abstract

We cloned a new imprinted gene by searching for parental-origin-specific CpG methylations using methylation-sensitive two-dimensional genome scanning method. This gene encodes a putative 51 kDa protein with significant similarity to U2 small nuclear ribonucleoprotein auxiliary factor small subunits, an essential mammalian splicing factor, and is located on mouse chromosome 11, of which maternal duplication/paternal deficiency results in a small body.

Related Genes
SP2
MeSH Terms
Amino Acid Sequence Animals Blotting, Northern Blotting, Southern Cloning, Molecular/methods DNA/genetics,metabolism Dinucleoside Phosphates/metabolism Genome Methylation Mice Mice, Inbred C57BL Mice, Inbred DBA Molecular Sequence Data Nerve Tissue Proteins/genetics Nuclear Proteins Polymerase Chain Reaction Restriction Mapping Ribonucleoproteins Ribonucleoproteins, Small Nuclear/genetics Splicing Factor U2AF
Chemicals
Dinucleoside Phosphates Nerve Tissue Proteins Nuclear Proteins Ribonucleoproteins Ribonucleoproteins, Small Nuclear Splicing Factor U2AF Zrsr1 protein, mouse cytidylyl-3'-5'-guanosine DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hatada I
National Cardiovascular Center Research Institute, Osaka, Japan.
Sugama T
Mukai T
References (18)
18 references, click to expand
  1. Genetic imprinting suggested by maternal heterodisomy in nondeletion Prader-Willi syndrome.
    Nature. 1989 Nov 16;342(6247):281-5 PMID: 2812027
  2. Differential imprinting and expression of maternal and paternal genomes.
    Annu Rev Genet. 1988;22:127-46 PMID: 3071246
  3. Genome imprinting phenomena on mouse chromosome 7.
    Genet Res. 1990 Oct-Dec;56(2-3):237-44 PMID: 2272514
  4. Parental imprinting of the mouse insulin-like growth factor II gene.
    Cell. 1991 Feb 22;64(4):849-59 PMID: 1997210
  5. Parental imprinting of the mouse H19 gene.
    Nature. 1991 May 9;351(6322):153-5 PMID: 1709450
  6. A genomic scanning method for higher organisms using restriction sites as landmarks.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9523-7 PMID: 1946366
  7. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.
    Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8769-73 PMID: 1388271
  8. Characterization of the spontaneous elimination of streptomycin sensitivity (SmS) on high-copy-number plasmids: SmS-enforcement cloning vectors with a synthetic rpsL gene.
    Gene. 1992 Nov 2;121(1):25-33 PMID: 1427096
  9. Maternal-specific methylation of the imprinted mouse Igf2r locus identifies the expressed locus as carrying the imprinting signal.
    Cell. 1993 Apr 9;73(1):61-71 PMID: 8462104
  10. Parental-origin-specific epigenetic modification of the mouse H19 gene.
    Nature. 1993 Apr 22;362(6422):751-5 PMID: 8469285
  11. Maternal imprinting of the mouse Snrpn gene and conserved linkage homology with the human Prader-Willi syndrome region.
    Nat Genet. 1992 Dec;2(4):259-64 PMID: 1303276
  12. A candidate mouse model for Prader-Willi syndrome which shows an absence of Snrpn expression.
    Nat Genet. 1992 Dec;2(4):270-4 PMID: 1303278
  13. Deletions of chromosome 15 as a cause of the Prader-Willi syndrome.
    N Engl J Med. 1981 Feb 5;304(6):325-9 PMID: 7442771
  14. Differential activity of maternally and paternally derived chromosome regions in mice.
    Nature. 1985 Jun 6-12;315(6019):496-8 PMID: 4000278
  15. Confidence limits for estimates of gene linkage based on analysis of recombinant inbred strains.
    J Hered. 1985 Nov-Dec;76(6):436-40 PMID: 4086787
  16. The molecular basis of the sparse fur mouse mutation.
    Science. 1987 Jul 24;237(4813):415-7 PMID: 3603027
  17. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  18. The mouse insulin-like growth factor type-2 receptor is imprinted and closely linked to the Tme locus.
    Nature. 1991 Jan 3;349(6304):84-7 PMID: 1845916
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.24.5577
Published
1993-12-11
Pages
5577-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310518
Subset
IM
Databases
GENBANK
D17407
External Links
PubMed source DOI: 10.1093/nar/21.24.5577 PMC Full Text View journal details
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