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PMID: 14656976 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Validation Study

A BAC-based physical map of the chicken genome.

Genome research ·Vol. 13 ·No. 12 ·2003-12-00 ·Pages 2754-8

Ren C, Lee MK, Yan B, Ding K, Cox B, Romanov MN, Price JA, Dodgson JB, Zhang HB

Abstract

A genome-wide physical map constructed with bacterial artificial chromosomes (BACs) is an essential component in linking phenotypic traits to the responsible genetic variation in the genomes of plants and animals. We have constructed a physical map of the chicken genome from 57,091 BACs (7.9-fold haploid genome coverage) by restriction fingerprint analysis using high-resolution polyacrylamide gel electrophoresis. The physical map consists of 2331 overlapping BAC contigs and is estimated to span 1510 Mb in physical length. BAC contigs were verified manually and by screening the BACs with 367 DNA markers. A total of 361 of the contigs have been anchored to the existing chicken genetic map. This map represents the first genome-wide, BAC-based physical map of the chicken genome. It provides a powerful platform for many areas of chicken genomics, including targeted marker development, fine mapping of genes and QTL alleles, positional cloning, analysis of avian genome organization and evolution, chicken-mammalian comparative genomics, and large-scale genome sequencing.

MeSH Terms
Animals Chickens/genetics Chromosomes, Artificial, Bacterial Computational Biology/methods Contig Mapping/methods DNA Fingerprinting Genome
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ren Chengwei
Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, Texas 77843, USA.
Lee Mi-Kyung
Yan Bo
Ding Kejiao
Cox Bettye
Romanov Michael N
Price Jennifer A
Dodgson Jerry B
Zhang Hong-Bin
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.1499303
Published
2003-12-00
Pages
2754-8
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC403818
Subset
IM
External Links
PubMed source DOI: 10.1101/gr.1499303 PMC Full Text View journal details
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