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PMID: 14649301 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

From protein structure to biochemical function?

Journal of structural and functional genomics ·Vol. 4 ·No. 2-3 ·2003-00-00 ·Pages 167-77

Laskowski RA, Watson JD, Thornton JM

Abstract

Here we describe various methods currently under development aimed at identifying a protein's function from its three-dimensional structure. We are combining a number of these methods to create a pipeline of applications, called ProFunc, which will take a given 3D structure, run all the applications on it and compile and summarise the results obtained. The aim is to provide a best guess as to the protein's function from the evidence provided by the different methods. Here we present three examples, using structures solved by the Midwest Center for Structural Genomics consortium, illustrating the strengths and weaknesses of current approaches.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/chemistry Bacterial Proteins/chemistry,metabolism Binding Sites Conserved Sequence Enzymes/chemistry,metabolism Escherichia coli Proteins/chemistry Ligands Methanobacterium/chemistry Models, Biological Models, Molecular Molecular Sequence Data Protein Conformation Protein Folding Software Structure-Activity Relationship Thermotoga maritima/chemistry
Chemicals
Bacterial Proteins CutA protein, E coli Enzymes Escherichia coli Proteins Ligands
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laskowski Roman A
European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Watson James D
Thornton Janet M
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Article Info
Journal
Journal of structural and functional genomics
Abbr.
J Struct Funct Genomics
ISSN
1345-711X
DOI
10.1023/a:1026127927612
Published
2003-00-00
Pages
167-77
Language
English
Region
Netherlands
NLM ID
101128185
Subset
IM
Grants
NIGMS NIH HHS · GM62414 · United States
External Links
PubMed source DOI: 10.1023/a:1026127927612
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