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PMID: 14602613 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Members of a readily enriched beta-proteobacterial clade are common in surface waters of a humic lake.

Applied and environmental microbiology ·Vol. 69 ·No. 11 ·2003-11-00 ·Pages 6550-9

Burkert U, Warnecke F, Babenzien D, Zwirnmann E, Pernthaler J

Abstract

Humic lakes are systems often characterized by irregular high input of dissolved organic carbon (DOC) from the catchment. We hypothesized that specific bacterial groups which rapidly respond to changes in DOC availability might form large populations in such habitats. Seasonal changes of microbial community composition were studied in two compartments of an artificially divided bog lake with contrasting DOC inputs. These changes were compared to community shifts induced during short-term enrichment experiments. Inocula from the two compartments were diluted 1:10 into water from the more DOC-rich compartment, and inorganic nutrients were added to avoid microbial N and P limitation. The dilutions were incubated for a period of 2 weeks. The microbial assemblages were analyzed by cloning and sequencing of 16S rRNA genes and by fluorescence in situ hybridization with specific oligonucleotide probes. beta-Proteobacteria from a cosmopolitan freshwater lineage related to Polynucleobacter necessarius (beta II) were rapidly enriched in all treatments. In contrast, members of the class Actinobacteria did not respond to the enhanced availability of DOC by an immediate increase in growth rate, and their relative abundances declined during the incubations. In lake water members of the beta II clade seasonally constituted up to 50% of all microbes in the water column. Bacteria from this lineage annually formed a significantly higher fraction of the microbial community in the lake compartment with a higher allochthonous influx than in the other compartment. Actinobacteria represented a second numerically important bacterioplankton group, but without clear differences between the compartments. We suggest that the pelagic microbial community of the studied system harbors two major components with fundamentally different growth strategies.

MeSH Terms
Betaproteobacteria/classification,genetics,growth & development Carbon/analysis Cloning, Molecular Colony Count, Microbial Culture Media Fresh Water/chemistry,microbiology Humic Substances In Situ Hybridization, Fluorescence Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Chemicals
Culture Media Humic Substances RNA, Ribosomal, 16S Carbon
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Burkert Ulrike
Leibniz Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany.
Warnecke Falk
Babenzien Dieter
Zwirnmann Elke
Pernthaler Jakob
References (26)
26 references, click to expand
  1. An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization.
    Appl Environ Microbiol. 2003 May;69(5):2928-35 PMID: 12732568
  2. Screening actinomycetes for extracellular peroxidase activity.
    Appl Environ Microbiol. 1996 Jun;62(6):2186-90 PMID: 16535344
  3. Predator-specific enrichment of actinobacteria from a cosmopolitan freshwater clade in mixed continuous culture.
    Appl Environ Microbiol. 2001 May;67(5):2145-55 PMID: 11319094
  4. Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of actinobacteria.
    Appl Environ Microbiol. 2000 Nov;66(11):5053-65 PMID: 11055963
  5. Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems.
    Appl Environ Microbiol. 1985 Mar;49(3):599-607 PMID: 3994368
  6. Are readily culturable bacteria in coastal North Sea waters suppressed by selective grazing mortality?
    Appl Environ Microbiol. 2003 May;69(5):2624-30 PMID: 12732530
  7. Phylogenetic relationships of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments.
    Arch Microbiol. 1995 Sep;164(3):165-72 PMID: 7545384
  8. Isolation of strains belonging to the cosmopolitan Polynucleobacter necessarius cluster from freshwater habitats located in three climatic zones.
    Appl Environ Microbiol. 2003 Sep;69(9):5248-54 PMID: 12957910
  9. Growth patterns of two marine isolates: adaptations to substrate patchiness?
    Appl Environ Microbiol. 2001 Sep;67(9):4077-83 PMID: 11526008
  10. Bacterioplankton Community Composition in Five Lakes Differing in Trophic Status and Humic Content.
    Microb Ecol. 2000 Aug;40(2):104-113 PMID: 11029079
  11. Marine Pseudoalteromonas species are associated with higher organisms and produce biologically active extracellular agents.
    FEMS Microbiol Ecol. 1999 Dec 1;30(4):285-293 PMID: 10568837
  12. Culturability and In situ abundance of pelagic bacteria from the North Sea.
    Appl Environ Microbiol. 2000 Jul;66(7):3044-51 PMID: 10877804
  13. Succession of pelagic marine bacteria during enrichment: a close look at cultivation-induced shifts.
    Appl Environ Microbiol. 2000 Nov;66(11):4634-40 PMID: 11055904
  14. Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization.
    Appl Environ Microbiol. 2000 Dec;66(12):5116-22 PMID: 11097877
  15. Effect of signal compounds and incubation conditions on the culturability of freshwater bacterioplankton.
    Appl Environ Microbiol. 2003 Apr;69(4):1980-9 PMID: 12676673
  16. Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
    Microbiol Rev. 1995 Mar;59(1):143-69 PMID: 7535888
  17. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
    J Mol Biol. 1981 May 15;148(2):107-27 PMID: 7028991
  18. Isolation of novel pelagic bacteria from the German bight and their seasonal contributions to surface picoplankton.
    Appl Environ Microbiol. 2001 Nov;67(11):5134-42 PMID: 11679337
  19. Determination of Active Marine Bacterioplankton: a Comparison of Universal 16S rRNA Probes, Autoradiography, and Nucleoid Staining.
    Appl Environ Microbiol. 1997 Apr;63(4):1208-13 PMID: 16535563
  20. Polynucleobacter necessarius, an obligate bacterial endosymbiont of the hypotrichous ciliate Euplotes aediculatus, is a member of the beta-subclass of Proteobacteria.
    FEMS Microbiol Lett. 1996 Jan 15;135(2-3):333-6 PMID: 8595875
  21. Isolation of novel ultramicrobacteria classified as actinobacteria from five freshwater habitats in Europe and Asia.
    Appl Environ Microbiol. 2003 Mar;69(3):1442-51 PMID: 12620827
  22. Fluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria.
    Appl Environ Microbiol. 2002 Jun;68(6):3094-101 PMID: 12039771
  23. Characterization of an extracellular lignin peroxidase of the lignocellulolytic actinomycete Streptomyces viridosporus.
    Appl Environ Microbiol. 1988 Dec;54(12):3057-63 PMID: 3223769
  24. Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. strain CCUG 15956.
    J Bacteriol. 1992 Nov;174(21):6780-8 PMID: 1383195
  25. Do neighboring lakes share common taxa of bacterioplankton? Comparison of 16S rDNA fingerprints and sequences from three geographic regions.
    Microb Ecol. 2002 Jul;44(1):1-9 PMID: 12016460
  26. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
    Appl Environ Microbiol. 1993 Mar;59(3):695-700 PMID: 7683183
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.69.11.6550-6559.2003
Published
2003-11-00
Pages
6550-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC262289
Subset
IM
Databases
GENBANK
AJ575497, AJ575498, AJ575499, AJ575500, AJ575501, AJ575502, AJ575503, AJ575504, AJ575505, AJ575506, AJ575507, AJ575508, AJ575509, AJ575510, AJ575511, AJ575512, AJ575513, AJ575514, AJ575515, AJ575516, AJ575517, AJ575518, AJ575519, AJ575520, AJ575521, AJ575522, AJ575523, AJ575524, AJ575525, AJ575526, AJ575527, AJ575528, AJ575529, AJ575530, AJ575531, AJ575532, AJ575533, AJ575534, AJ575535, AJ575536, AJ575537, AJ575538, AJ575539, AJ575540, AJ575541, AJ575542, AJ575543, AJ575544, AJ575545, AJ575546, AJ575547, AJ575548, AJ575549, AJ575550, AJ575551, AJ575552, AJ575553, AJ575554, AJ575555, AJ575556
External Links
PubMed source DOI: 10.1128/AEM.69.11.6550-6559.2003 PMC Full Text View journal details
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