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PMID: 1438293 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Retinal rods and cones have distinct G protein beta and gamma subunits.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 22 ·1992-11-15 ·Pages 10882-6

Peng YW, Robishaw JD, Levine MA, Yau KW

Abstract

Guanine nucleotide-binding proteins (G proteins) involved in transmembrane signal-transduction processes are heterotrimers composed of alpha, beta, and gamma subunits. The alpha subunit shows great diversity and is thought to confer functional specificity to a particular G protein. By contrast, the beta and gamma subunits appear much less diverse; in particular, the beta subunit is believed to have no role in G protein specificity. Using immunocytochemistry, we found distinct distribution patterns for different beta and gamma subunits in the retina. In particular, rod and cone photoreceptors, which both subserve phototransduction but differ in light-response properties, have different beta and gamma subunits in their outer segments. Thus, the G protein mediating phototransduction shows cell-specific forms of the beta and gamma subunits in addition to the alpha subunit. This surprising finding supports the hypothesis that these subunits may also contribute to functional specificity of a G protein.

MeSH Terms
Amino Acid Sequence Animals Antibodies GTP-Binding Proteins/analysis,immunology Immunoenzyme Techniques Immunohistochemistry Macaca fascicularis Macromolecular Substances Molecular Sequence Data Peptides/chemical synthesis,immunology Photoreceptor Cells/cytology Retina/cytology
Chemicals
Antibodies Macromolecular Substances Peptides GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Peng Y W
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Robishaw J D
Levine M A
Yau K W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.22.10882
Published
1992-11-15
Pages
10882-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50446
Subset
IM
Grants
NIDDK NIH HHS · DK34281 · United States
NEI NIH HHS · EY06837 · United States
NIGMS NIH HHS · GM39867 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.22.10882 PMC Full Text View journal details
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