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PMID: 14316942 Published · ppublish English Journal Article

STUDIES ON TUBERCULIN FEVER. 3. MECHANISMS INVOLVED IN THE RELEASE OF ENDOGENOUS PYROGEN IN VITRO.

The Journal of experimental medicine ·Vol. 122 ·1965-08-01 ·Pages 207-35

ATKINS E, HEIJN C

Abstract

In a search for the source of the circulating endogenous pyrogen (EP) that mediates tuberculin-induced fever, tuberculin was incubated in vitro with various tissues of rabbits sensitized by intravenous infection with BCG. Evidence was obtained that tuberculin specifically stimulates cells in the blood of sensitized rabbits to generate pyrogen in vitro, whereas both lymph node and spleen cells from the same donors were inactive. Since normal blood cells, incubated in plasma of sensitized donors, were similarly activated, it is postulated that circulating antibodies play a role in sensitizing cells (presumably granulocytes) to release pyrogen on contact with tuberculin) both in vitro and in vivo. Release of endogenous pyrogen in vitro may be a sensitive means of detecting immunologic reactions between antigen and specifically sensitized blood cells-in other allergic states accompanied by fever.

Keywords
BLOOD CELLS EXPERIMENTAL LAB STUDY FEVER HYPERSENSITIVITY IMMUNOLOGY LEUKOCYTES LYMPH NODES LYMPHOCYTES PYROGENS RABBITS SPLEEN TUBERCULIN
MeSH Terms
Allergy and Immunology Animals Antibodies Antigens Blood Cells Fever Hypersensitivity In Vitro Techniques Interleukin-1 Leukocytes Lymph Nodes Lymphocytes Pyrogens Rabbits Research Spleen Tuberculin
Chemicals
Antibodies Antigens Interleukin-1 Pyrogens Tuberculin leukocyte endogenous mediator
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
ATKINS E
HEIJN C
References (32)
32 references, click to expand
  1. EFFECTS OF MITOMYCIN C ON THE CELLULAR TRANSFER OF DELAYED-TYPE HYPERSENSITIVITY IN THE GUINEA PIG.
    Nature. 1964 Feb 15;201:689-91 PMID: 14134712
  2. Studies on the pathogenesis of fever. XI. Quantitative features of the febrile response to leucocytic pyrogen.
    J Exp Med. 1963 Mar 1;117:349-64 PMID: 14014022
  3. Studies on the pathogenesis of fever. X. The effect of certain enzyme inhibitors on the production and activity of leucocvtic pvrogen.
    J Exp Med. 1962 Jan 1;115:37-47 PMID: 14453158
  4. Molecular mechanisms involved in the release of pyrogen from polymorphonuclear leucocytes.
    Trans Assoc Am Physicians. 1962;75:190-7 PMID: 13970889
  5. In vitro effect of tuberculin on cells.
    Bibl Tuberc. 1951;5:219-35 PMID: 13018098
  6. Analysis of leukocytic extracts from guinea pigs hypersensitive to tuberculin and 2,4-dinitrochlorobenzene.
    J Bacteriol. 1957 Nov;74(5):680-3 PMID: 13481009
  7. Cell injury in allergic inflammation.
    Int Arch Allergy Appl Immunol. 1957;10(4):193-224 PMID: 13474810
  8. STUDIES ON THE PATHOGENESIS OF FEVER. XII. ELECTROLYTIC FACTORYS INFLUENCING THE RELEASE OF ENDOGENOUS PYROGEN FROM POLYMORPHONUCLEAR LEUKOCYTES.
    J Exp Med. 1964 May 1;119:697-714 PMID: 14159044
  9. Studies on the pathogenesis of fever. II. Characterization of fever-producing substances from polymorphonuclear leukocytes and from the fluid of sterile exudates.
    J Exp Med. 1953 Nov;98(5):493-508 PMID: 13109105
  10. Specific serum agglutination of erythrocytes sensitized with extracts of tubercle bacilli.
    J Exp Med. 1948 Nov;88(5):521-8 PMID: 18892427
  11. Studies on the pathogenesis of fever. I. The effect of injection of extracts and suspensions of uninfected rabbit tissues upon the body temperature of normal rabbits.
    J Exp Med. 1953 Nov;98(5):477-92 PMID: 13109104
  12. Induction of 'delayed' hypersensitivity.
    Nature. 1962 Feb 3;193:492-3 PMID: 14482145
  13. TRANSFER OF CUTANEOUS HYPERSENSITIVITY TO TUBERCULIN WITH A CELL-FREE EXTRACT OF SPLENIC TISSUE.
    Proc Soc Exp Biol Med. 1964 Feb;115:374-9 PMID: 14121614
  14. Occurrence of delayed hypersensitivity during the development of Arthus type hypersensitivity.
    J Exp Med. 1958 Jan 1;107(1):109-24 PMID: 13481259
  15. The role of leucocytes in the initial action of bacterial pyrogens in man.
    Clin Sci. 1956 May;15(2):219-26 PMID: 13356552
  16. ON THE CELLULAR FIXATION OF CYTOPHILIC ANTIBODY.
    Int Arch Allergy Appl Immunol. 1964;25:129-44 PMID: 14219619
  17. ENDOGENOUS PYROGEN RELEASE FROM RABBIT BLOOD CELLS INCUBATED IN VITRO WITH PARAINFLUENZA VIRUS.
    Science. 1964 Dec 11;146(3650):1469-70 PMID: 14208572
  18. THE PRESENCE OF ENDOGENOUS PYROGEN IN NORMAL RABBIT TISSUES.
    J Exp Med. 1965 Jun 1;121:1019-38 PMID: 14319400
  19. Hypersensitivity of the delayed type.
    Harvey Lect. 1956-1958;(Series 52):100-18 PMID: 13512852
  20. STUDIES ON THE "TRANSFER FACTOR" OF TUBERCULIN HYPERSENSITIVITY IN ANIMALS. I. OBSERVATION OF SUCCESSFUL PASSIVE TRANSFER OF TUBERCULIN HYPERSENSITIVITY WITH FRACTIONS OF EITHER DISRUPTED ALVEOLAR MACROPHAGES OR SERUM OF SENSITIZED AND CHALLENGED RABBITS.
    J Immunol. 1964 Nov;93:838-49 PMID: 14239944
  21. CYTOPHILIC ANTIBODY IN GUINEA-PIGS WITH DELAYED-TYPE HYPERSENSITIVITY.
    Immunology. 1964 Jul;7:474-83 PMID: 14193159
  22. CELL-BOUND AND CELL-BINDING ANTIBODY IN THE PERIPHERAL BLOOD OF IMMUNIZED RABBITS.
    Nature. 1964 Nov 21;204:794-5 PMID: 14235686
  23. Studies on tuberculin fever. II. Observations on the role of endogenous pyrogen in tolerance.
    J Exp Med. 1961 Dec 1;114:939-59 PMID: 14476462
  24. The immunological response to antigens of the tubercle bacillus; some experimental aspects.
    Prog Allergy. 1958;5:149-214 PMID: 13578998
  25. Pathogenesis of fever.
    Physiol Rev. 1960 Jul;40:580-646 PMID: 13794961
  26. In vivo uptake of isotope-tagged tuberculin by leukocytes.
    J Immunol. 1955 Sep;75(3):209-13 PMID: 13252263
  27. Erythrocyte destruction in tuberculous animals following tuberculin injection.
    J Infect Dis. 1951 Jan-Feb;88(1):9-16 PMID: 14803744
  28. The delayed type of allergic inflammatory response.
    Am J Med. 1956 Mar;20(3):428-47 PMID: 13292452
  29. Fixation of bacterial products by erythrocytes in vivo and by Leucocytes.
    Nature. 1953 Feb 28;171(4348):402-3 PMID: 13036913
  30. Studies on the pathogenesis of fever. VI. The interaction of leucocytes and endotoxin in vitro.
    J Exp Med. 1959 Dec 1;110:1005-16 PMID: 13811296
  31. STUDIES ON THE PATHOGENESIS OF FEVER. 13. THE EFFECT OF PHAGOCYTOSIS ON THE RELEASE OF ENDOGENOUS PYROGEN BY POLYMORPHONUCLEAR LEUKOCYTES.
    J Exp Med. 1964 May 1;119:715-26 PMID: 14157026
  32. Studies on tuberculin fever. I. The mechanism of fever in tuberculin hypersensitivity.
    J Exp Med. 1959 Apr 1;109(4):339-59 PMID: 13641561
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.122.2.207
Published
1965-08-01
Pages
207-35
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138054
Subset
OM
External Links
PubMed source DOI: 10.1084/jem.122.2.207 PMC Full Text View journal details
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