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PMID: 13811296 Published · ppublish English Journal Article

Studies on the pathogenesis of fever. VI. The interaction of leucocytes and endotoxin in vitro.

The Journal of experimental medicine ·Vol. 110 ·1959-12-01 ·Pages 1005-16

COLLINS RD, WOOD WB

Abstract

Study in vitro of the interaction of bacterial endotoxin with rabbit polymorphonuclear leucocytes has resulted in the following findings: 1. Incubation of endotoxin and leucocytes in saline results in: (a) the release of leucocytic pyrogen, and (b) the inactivation of endotoxin. 2. Cell-free extracts of leucocytes also inactivate endotoxin. 3. Incubation of leucocytes in "physiological" saline causes rapid discharge of leucocytic pyrogen. In contrast, relatively little pyrogen is released by leucocytes incubated in fresh serum. 4. The release of leucocytic pyrogen in serum is markedly stimulated by the presence of endotoxin. 5. Leucocytes obtained from tolerant rabbits interact with endotoxin in essentially the same manner as leucocytes from normal rabbits. The pertinence of these findings to the pathogenesis of fever and to related information concerning human leucocytes has been discussed.

Keywords
FEVER/physiology LEUKOCYTES/physiology
MeSH Terms
Animals Endotoxins Fever/physiology In Vitro Techniques Leukocytes/physiology Pyrogens Rabbits
Chemicals
Endotoxins Pyrogens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
COLLINS R D
WOOD W B
References (20)
20 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.110.6.1005
Published
1959-12-01
Pages
1005-16
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2137036
Subset
OM
External Links
PubMed source DOI: 10.1084/jem.110.6.1005 PMC Full Text View journal details
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