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PMID: 141463 Published · ppublish English Journal Article

Relationship between bile flow and Na+, K+-adenosinetriphosphatase in liver plasma membranes enriched in bile canaliculi.

The Journal of clinical investigation ·Vol. 60 ·No. 2 ·1977-08-00 ·Pages 429-34

Reichen J, Paumgartner G

Abstract

The relationship between bile flow and Na+,K+-ATPase activity in liver plasma membranes enriched in bile canaliculi was studied in rats treated with ethinyl estradiol, phenobarbital, or 20-methyl cholanthrene. In comparison with controls (1.49+/-0.12 microliter/min per g liver), bile flow was significantly diminished by ethinyl estradiol, increased by phenobarbital, and unchanged by 20-methyl cholanthrene or the solvent, propanediol (0.92+/-0.31, 2.50+/-0.21, 1.62+/-0.18, and 1.64+/-0.30 microliter/min per g liver, respectively). The corresponding values for canalicular Na+,K+-ATPase activity were 80.7+/-19.2, 50.0+/-18.4, 231.7+/-42.6, 82.7+/-30.7, and 143.6+/-55.3 micronmol Pi/h per g liver. Canalicular Na+,K+-ATPase activity was significantly correlated (r=0.785, n=31) with bile flow. These findings support the hypothesis that a fraction of bile flow is related to Na+,K+-ATPase activity and canalicular Na+ transport.

MeSH Terms
Adenosine Triphosphatases/metabolism Animals Bile/physiology Body Weight Cell Membrane/enzymology Enzyme Activation Ethinyl Estradiol/pharmacology Gallbladder/metabolism,ultrastructure Liver/drug effects,enzymology Male Methylcholanthrene/pharmacology Microscopy, Electron Organ Size Phenobarbital/pharmacology Potassium/metabolism Propylene Glycols/pharmacology Rats Sodium/metabolism
Chemicals
Propylene Glycols Ethinyl Estradiol Methylcholanthrene Sodium Adenosine Triphosphatases Potassium Phenobarbital
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reichen J
Paumgartner G
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27 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108792
Published
1977-08-00
Pages
429-34
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC372384
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108792 PMC Full Text View journal details
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