Home LiteratureArticle Details
PMID: 4265000 Published · ppublish English Journal Article

Effects of phenobarbital and rose bengal on the ATPases of plasma membranes of rat and rabbit liver.

Gut ·Vol. 13 ·No. 11 ·1972-11-00 ·Pages 920-5

Laperche Y, Launay A, Oudéa P

Abstract

The effects of drugs which change the bile-salt-independent fraction of bile flow on Na(+)K(a+) and Mg(2+) activated ATPases were studied in membrane fractions rich in bile canaliculi. The administration of phenobarbital caused no induction of these enzymes which could explain the increased bile flow observed in the rat. Rose bengal, in addition to its strong photoxidative inhibition of both ATPases, inhibits the Na(+)K(+) ATPase of rat and rabbit bile canaliculi in the absence of light. A closely related substance, uranine, inhibits neither bile flow nor Na(+)K(+)ATPase. Inhibition of this enzyme by rose bengal may therefore be responsible for the observed effects of this dye on bile flow independent of bile salts.

MeSH Terms
Adenosine Triphosphatases/analysis,antagonists & inhibitors Animals Bile/drug effects Cell Membrane/drug effects,enzymology Liver/enzymology Magnesium/pharmacology Male Microscopy, Electron Oxidation-Reduction Phenobarbital/pharmacology Potassium/pharmacology Rabbits Rats Rose Bengal/pharmacology Sodium/pharmacology
Chemicals
Rose Bengal Sodium Adenosine Triphosphatases Magnesium Potassium Phenobarbital
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laperche Y
Launay A
Oudéa P
References (21)
21 references, click to expand
  1. Biliary flow after microsomal enzyme induction.
    J Pharmacol Exp Ther. 1969 Aug;168(2):218-23 PMID: 4389885
  2. Nonphotooxidative eosin Y inhibition of Na+-K+ ATPase activity in the human erythrocyte membrane.
    Arch Biochem Biophys. 1971 Jul;145(1):43-9 PMID: 4256443
  3. Studies on plasma membranes. 3. Mg2+-ATPase,(Na+-K+-Mg2+)-ATPase and 5'-nucleotidase activity of plasma membranes isolated from rat liver.
    Biochim Biophys Acta. 1966 Jul 13;120(3):369-82 PMID: 4226064
  4. Increased bilirubin conjugation in heterozygous Gunn rats treated with phenobarbital.
    Nature. 1969 Jun 7;222(5197):990-1 PMID: 5789331
  5. Purification of plasma membranes of rat liver. Application of zonal centrifugation to isolation of cell membranes.
    Biochim Biophys Acta. 1969 Apr;173(3):377-88 PMID: 5769638
  6. Effect of inhibitors of sodium transport on bile formation in the rabbit.
    Am J Physiol. 1970 Aug;219(2):416-22 PMID: 5448072
  7. Effects of phenobarbital on biliary excretion of organic acids in male and female rats.
    Am J Physiol. 1969 Jul;217(1):46-52 PMID: 5785898
  8. Mechanism of phenobarbital-induced hypercholeresis in the rat.
    Am J Physiol. 1970 Sep;219(3):809-13 PMID: 5450891
  9. Canalicular bile formation in the isolated perfused rat liver.
    Am J Physiol. 1971 Oct;221(4):1156-63 PMID: 4255749
  10. Sodium-stimulated adenosinetriphosphatase in microsomes from rat brain.
    Biochim Biophys Acta. 1961 Mar 18;48:104-10 PMID: 13789564
  11. Lipid class and fatty acid composition of rat liver plasma membranes isolated by zonal centrifugation.
    Biochemistry. 1968 Aug;7(8):2826-33 PMID: 4299085
  12. Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.
    Biochem J. 1961 Sep;80:649-54 PMID: 13734134
  13. Fome properties of a kidney adenosine triphosphatase relevant to active cation transport.
    Biochem J. 1962 Dec;85:495-507 PMID: 14000239
  14. A purified plasma membrane fraction isolated from rat liver under isotonic conditions.
    Biochim Biophys Acta. 1967 Sep 9;135(4):573-9 PMID: 4292717
  15. Effect on bile formation of inhibitors of sodium transport.
    Nature. 1969 Sep 20;223(5212):1276-7 PMID: 5807001
  16. Preparation of a plasma membrane fraction from rat liver by zonal centrifugation.
    Eur J Biochem. 1970 Feb;12(2):349-59 PMID: 4318905
  17. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  18. Permeability and photoxidative damage of membrane enzymes.
    J Cell Physiol. 1969 Dec;74(3):259-72 PMID: 4243143
  19. Effects of rose bengal on bile secretion in the rabbit: inhibition of a bile salt-independent fraction.
    Gut. 1970 Feb;11(2):134-40 PMID: 5441882
  20. Effect of phenobarbital on the excretion of an exogenous bilirubin load.
    Biochem Pharmacol. 1967 May;16(5):827-35 PMID: 6036733
  21. Cation exchanges and glycolytic inhibition by photosensitized rabbit erythrocytes.
    J Cell Comp Physiol. 1962 Apr;59:215-22 PMID: 13871295
Article Info
Journal
Gut
Abbr.
Gut
ISSN
0017-5749
DOI
10.1136/gut.13.11.920
Published
1972-11-00
Pages
920-5
Language
English
Region
England
NLM ID
2985108R
PMCID
PMC1412415
Subset
IM
External Links
PubMed source DOI: 10.1136/gut.13.11.920 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2