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PMID: 14115 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of an Escherichia coli mutant deficient in thioredoxin reductase.

Journal of bacteriology ·Vol. 129 ·No. 2 ·1977-02-00 ·Pages 967-72

Fuchs J

Abstract

A mutant of Escherichia coli defective in thioredoxin reductase has been isolated and partially characterized. This mutant has no detectable thioredoxin reductase activity in vitro and yet it exhibits no in vivo defect in reduction of ribonucleotides. Evidence is presented that indicates that, in cells permeabilized via ether treatment, ribonucleoside diphosphate reduction can utilize glutathione as an alternate reducing system.

MeSH Terms
Escherichia coli/enzymology,metabolism Glutathione/metabolism Methionine/metabolism Methionine Sulfoximine/metabolism Mutation NADH, NADPH Oxidoreductases/biosynthesis NADP/metabolism Ribonucleoside Diphosphate Reductase/metabolism Temperature Thioredoxin-Disulfide Reductase/biosynthesis,metabolism Thioredoxins/metabolism Uracil Nucleotides/metabolism
Chemicals
Uracil Nucleotides Methionine Sulfoximine Thioredoxins NADP Methionine Ribonucleoside Diphosphate Reductase NADH, NADPH Oxidoreductases Thioredoxin-Disulfide Reductase Glutathione
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Fuchs J
References (22)
22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.129.2.967-972.1977
Published
1977-02-00
Pages
967-72
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235035
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.129.2.967-972.1977 PMC Full Text View journal details
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