OXAMIC TRANSCARBAMYLASE OF STREPTOCOCCUS ALLANTOICUS.

Journal of bacteriology ·Vol. 87 ·1964-01-00 ·Pages 75-80

BOJANOWSKI R, GAUDY E, VALENTINE RC, WOLFE RS

Abstract

Bojanowski, R. (University of Illinois, Urbana), Elizabeth Gaudy, R. C. Valentine, and R. S. Wolfe. Oxamic transcarbamylase of Streptococcus allantoicus. J. Bacteriol. 87:75-80. 1964.-An improved colorimetric assay for carbamyl oxamate, which allows the precise measurement of the activity of oxamic transcarbamylase, has been developed. Activity is maximum over the pH range from 8.3 to 8.7. A cation requirement is satisfied by 2.5 x 10(-3)m Mg(++) or Mn(++). The equilibrium constant for the phosphorolysis of carbamyl oxamic acid is 1.6, corresponding to a negative free energy change of -285 cal per mole.

Keywords

ADENOSINE TRIPHOSPHATE AMINO ACIDS COLORIMETRY EXPERIMENTAL LAB STUDY MAGNESIUM MANGANESE ORNITHINE PROTEINS STREPTOCOCCUS TRANSFERASES

MeSH Terms

Adenosine Triphosphate Amino Acids Carboxyl and Carbamoyl Transferases Colorimetry Ions Magnesium Manganese Ornithine Proteins Research Streptococcus Transferases

Chemicals

Amino Acids Ions Proteins Manganese Adenosine Triphosphate Ornithine Transferases Carboxyl and Carbamoyl Transferases oxamate carbamoyltransferase Magnesium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

BOJANOWSKI R

GAUDY E

VALENTINE R C

WOLFE R S

References (7)

7 references, click to expand

Phosphorolysis of carbamyl oxamic acid.
Biochim Biophys Acta. 1960 Dec 4;45:389-91 PMID: 13779744
Streptococcus allantoicus and the Fermentation of Allantoin.
J Bacteriol. 1943 Sep;46(3):251-9 PMID: 16560697
A survey of the energy transformations in living matter.
Ergeb Physiol. 1957;49:212-98 PMID: 13609573
Colorimetric determination of carbamylamino acids and related compounds.
J Biol Chem. 1954 Jul;209(1):145-50 PMID: 13192067
Synthesis of citrulline with animal and bacterial enzymes.
J Biol Chem. 1960 Mar;235:684-93 PMID: 13807603
Mechanism of the allantoin fermentation.
J Biol Chem. 1962 Jul;237:2271-7 PMID: 13924343
[Determination of N-carbamoyl derivatives of amino acids by diacetylmonoxime].
Bull Soc Chim Biol (Paris). 1958;40(7-8):1093-106 PMID: 13596813

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.87.1.75-80.1964

Published

1964-01-00

Pages

75-80

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC276964

Subset

External Links

PubMed source DOI: 10.1128/jb.87.1.75-80.1964 PMC Full Text View journal details

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