Mutations in the cdc10 start gene of Schizosaccharomyces pombe implicate the region of homology between cdc10 and SWI6 as important for p85cdc10 function.

Molecular & general genetics : MGG ·Vol. 234 ·No. 3 ·1992-09-00 ·Pages 449-56

Reymond A, Schmidt S, Simanis V

Abstract

The cdc10 gene of the fission yeast Schizosaccharomyces pombe is required for traverse of start and commitment to the mitotic cell division cycle rather than other fates. The product of the gene, p85cdc10, is a component of a factor that is thought to be involved in regulating the transcription of genes that are required for DNA synthesis. In order to define regions of the p85cdc10 protein that are important for its function a fine structure genetic map of the cdc10 gene was derived and the sequences of 13 cdc10ts mutants determined. The 13 mutants tested define eight alleles. Eleven of the mutants are located in the region that contains the two copies of the cdc10/SWI6 repeat motif, implicating it as important for p85cdc10 function.

Related Genes

SWI6 cdc10

MeSH Terms

Amino Acid Sequence Base Sequence Cell Cycle Cell Cycle Proteins Chromosome Mapping DNA Mutational Analysis Fungal Proteins/genetics GTP Phosphohydrolases Genes, Fungal Membrane Proteins Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Polymerase Chain Reaction Saccharomyces cerevisiae Proteins Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins Sequence Alignment Structure-Activity Relationship Transcription Factors/genetics

Chemicals

Cell Cycle Proteins Fungal Proteins Membrane Proteins Oligodeoxyribonucleotides SWI6 protein, S cerevisiae Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Transcription Factors cdc10 protein, S pombe CDC10 protein, S cerevisiae GTP Phosphohydrolases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Reymond A

Unité de Recherche sur le cycle cellulaire, ISREC, Epalinges, Switzerland.

Schmidt S

Simanis V

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00538705

Published

1992-09-00

Pages

449-56

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00538705

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Mutations in the cdc10 start gene of Schizosaccharomyces pombe implicate the region of homology between cdc10 and SWI6 as important for p85cdc10 function.

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