Cloning and structure of cDNA encoding alpha-latrotoxin from black widow spider venom.

FEBS letters ·Vol. 270 ·No. 1-2 ·1990-09-17 ·Pages 127-31

Kiyatkin NI, Dulubova IE, Chekhovskaya IA, Grishin EV

Abstract

cDNA encoding the putative alpha-latrotoxin precursor was isolated from spider venom glands cDNA library and sequenced. The cDNA contained the 4203 base-pair open reading frame corresponding to the 156,855-Da protein composed of 1401 amino acids. Computer analysis of the deduced primary structure revealed the presence of various internal imperfect repeats mainly in its central and C-terminal regions.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cloning, Molecular Gene Library Molecular Sequence Data Peptide Fragments/genetics Poly A/genetics RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Spider Venoms/chemistry,genetics

Chemicals

Peptide Fragments RNA, Messenger Spider Venoms Poly A alpha-latrotoxin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kiyatkin N I

Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.

Dulubova I E

Chekhovskaya I A

Grishin E V

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(90)81250-r

Published

1990-09-17

Pages

127-31

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(90)81250-r View journal details

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Cloning and structure of cDNA encoding alpha-latrotoxin from black widow spider venom.

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