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PMID: 1398766 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transforming growth factor-beta enhances interleukin-6 secretion by intestinal epithelial cells.

Immunology ·Vol. 77 ·No. 1 ·1992-09-00 ·Pages 7-12

McGee DW, Beagley KW, Aicher WK, McGhee JR

Abstract

Recent reports have suggested that transforming growth factor-beta (TGF-beta) may have an important role in IgA immune responses, e.g. induction of surface IgM+ B cells to commit to IgA. TGF-beta is also an important regulatory cytokine for the maturation of intestinal epithelial cells. Using the IEC-6 rat intestinal epithelial cell line as a model system, TGF-beta 1 was found to enhance interleukin-6 (IL-6) secretion by the IEC-6 cells. The IL-6 was produced in a dose-dependent manner and secretion could be specifically inhibited by an anti-TGF-beta 1 antibody. IL-6 production by the IEC-6 cells was confirmed by using a rabbit anti-mouse IL-6 antibody which completely neutralized the IL-6 present in the IEC-6 cell supernatant. The enhancement of IL-6 secretion was found to involve a low-level enhancement in the expression of RNA for IL-6. The induction of IL-6 secretion was also reversible when TGF-beta was removed. These results suggest that the action of TGF-beta on intestinal epithelial cells may play an important role in immune responses at the intestinal mucosa.

MeSH Terms
Animals Cell Line Epithelium/immunology Interleukin-6/biosynthesis,genetics,immunology Intestinal Mucosa/immunology RNA, Messenger/biosynthesis Rats Time Factors Transforming Growth Factor beta/immunology
Chemicals
Interleukin-6 RNA, Messenger Transforming Growth Factor beta
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McGee D W
Department of Microbiology, University of Alabama, Birmingham 35294.
Beagley K W
Aicher W K
McGhee J R
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1992-09-00
Pages
7-12
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1421595
Subset
IM
Grants
NIAID NIH HHS · AI 18958 · United States
NIAID NIH HHS · AI 19674 · United States
NIDCR NIH HHS · DE 04217 · United States
External Links
PubMed source PMC Full Text View journal details
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