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PMID: 13972781 Published · ppublish English Journal Article

Continuous culture of ruminal microorganisms in chemically defined medium. I. Design of continuous-culture apparatus.

Applied microbiology ·Vol. 10 ·1962-11-00 ·Pages 580-2

QUINN LY

Abstract

An apparatus is described which has been used for successful continuous culture of the ciliates from the rumen of cattle. Automatic control of feeding rate, pH, oxidation-reduction potential, temperature, stirring rate, aeration rate, salinity, and volume of the culture is provided for, using standard commercial equipment, whenever possible. The operation of this apparatus is described.

Keywords
BACTERIOLOGICAL TECHNICS CILIATA RUMEN
MeSH Terms
Animals Bacteriological Techniques Cattle Ciliophora Gadiformes Rumen
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
QUINN L Y
References (15)
15 references, click to expand
  1. The cultivation and metabolism of oligotrich Protozoa from the sheep's rumen.
    J Gen Microbiol. 1953 Aug;9(1):44-53 PMID: 13084877
  2. A new method for the study in vitro of rumen digestion.
    Science. 1949 Nov 4;110(2862):478-80 PMID: 15392953
  3. Fermentation of soluble sugars by anaerobic holotrich ciliate protozoa of the genera Isotricha and Dasytricha.
    Biochem J. 1953 Feb;53(3):506-12 PMID: 13032102
  4. Some cultural studies with holotrich ciliate protozoa of the sheep's rumen.
    J Gen Microbiol. 1952 Aug;7(1-2):145-53 PMID: 13011266
  5. Maintenance of oligotrich protozoa from the sheep rumen in vitro.
    Nature. 1958 Oct 18;182(4642):1104-5 PMID: 13590247
  6. Continuous culture of ruminal microorganisms in chemically defined medium. II. Culture medium studies.
    Appl Microbiol. 1962 Nov;10:583-92 PMID: 13972780
  7. Bacterial culture with controlled pH.
    Appl Microbiol. 1955 Nov;3(6):355-60 PMID: 13269088
  8. Symposium: selected topics in microbial ecology. I. Microbial ecology of the rumen.
    Bacteriol Rev. 1960 Dec;24(4):353-64 PMID: 13716827
  9. THE SIGNIFICANCE OF THE BACTERIA AND THE PROTOZOA OF THE RUMEN OF THE BOVINE .
    Bacteriol Rev. 1944 Dec;8(4):235-54 PMID: 16350096
  10. Criteria for establishing the validity of in vitro studies with rumen micro-organisms in so-called artificial rumen systems.
    J Gen Microbiol. 1956 Jul;14(3):733-48 PMID: 13346034
  11. Experiments on the culture and physiology of holotriches from the bovine rumen.
    Biochem J. 1955 Jul;60(3):516-22 PMID: 13239588
  12. Growth of rumen microorganisms in an in vitro continuous-flow system on a protein-free diet.
    Cornell Vet. 1958 Jan;48(1):53-66 PMID: 13490038
  13. The rumen ciliate protozoa: their chemical composition, metabolism, requirements for maintenance and culture, and physiological significance for the host.
    Exp Parasitol. 1955 Nov;4(6):569-605 PMID: 13277528
  14. Continuous Culture as a Method for Studying Rumen Fermentation.
    Appl Microbiol. 1961 Mar;9(2):150-6 PMID: 16349600
  15. The cultivation of sheep rumen oligotrich protozoa in vitro.
    J Gen Microbiol. 1960 Apr;22:555-63 PMID: 13811149
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.10.6.580-582.1962
Published
1962-11-00
Pages
580-2
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC1057919
Subset
OM
External Links
PubMed source DOI: 10.1128/am.10.6.580-582.1962 PMC Full Text
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