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PMID: 1394509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic mapping of 1,3-beta-glucanase-encoding genes in Saccharomyces cerevisiae.

Current genetics ·Vol. 22 ·No. 4 ·1992-10-00 ·Pages 283-8

Correa J, Vazquez de Aldana CR, San Segundo P, del Rey F

Abstract

The map position of three 1,3-beta-glucanase-encoding genes in S. cerevisiae has been determined following conventional meiotic and mitotic mapping combined with recombinant DNA techniques. EXG1, EXG2 and SSG1 were localized to chromosomes XII, IV and XV, respectively, by hybridizing the cloned genes to Southern blots of chromosomes separated by pulsed-field gel electrophoresis, in conjunction with the rad52-1-dependent chromosome-loss mapping technique. Meiotic tetrad analyses further localized the EXG1 gene 6.1 centimorgans centromere-proximal to CDC25 on the right arm of chromosome XII. EXG2 was positioned between LYS4 and GCN2 on the right arm of chromosome IV, at distances of 6.2 centimorgans from LYS4 and 4.9 centimorgans from GCN2. Finally, the SSG1 locus mapped on the right arm of chromosome XV, about 8.2 centimorgans to the centromere-proximal side of HIS3.

MeSH Terms
Chromosome Mapping/methods Chromosomes, Fungal Cloning, Molecular Fungal Proteins/genetics Genes, Fungal Genetic Linkage Glucan 1,3-beta-Glucosidase Meiosis Saccharomyces cerevisiae/genetics beta-Glucosidase/genetics
Chemicals
Fungal Proteins beta-Glucosidase Glucan 1,3-beta-Glucosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Correa J
Instituto de Microbiología-Bioquímica, Facultad de Biología, CSIC-Universidad de Salamanca, Spain.
Vazquez de Aldana C R
San Segundo P
del Rey F
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00317922
Published
1992-10-00
Pages
283-8
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00317922 View journal details
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