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PMID: 1384266 Published · ppublish English Journal Article

Monoclonal antibodies with selective specificity for Alzheimer Tau are directed against phosphatase-sensitive epitopes.

Acta neuropathologica ·Vol. 84 ·No. 3 ·1992-00-00 ·Pages 265-72

Mercken M, Vandermeeren M, Lübke U, Six J, Boons J, Van de Voorde A, Martin JJ, Gheuens J

Abstract

A modified form of the microtubule-associated protein Tau is the major component of the paired helical filaments (PHF) found in Alzheimer's disease. The characterization of these posttranslational Tau modifications is hindered by the lack of sufficient PHF-Tau-specific markers. Here we describe several monoclonal antibodies, prepared by immunization with PHF, two of which showed a selective specificity for PHF-Tau without cross-reactivity with normal Tau. Epitope recognition by these two monoclonals was sensitive to alkaline phosphatase treatment. In Western blotting these monoclonal antibodies reacted specifically with the abnormally phosphorylated epitopes on Alzheimer's disease-associated PHF-Tau. One of the new antibodies can be used for the construction of a sandwich enzyme-linked immunosorbent assay for the specific detection of PHF-Tau without cross-reactivity to normal Tau proteins.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Blotting, Western Cross Reactions Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitopes/immunology Humans Mice Mice, Inbred BALB C Neurofilament Proteins/immunology Phosphoric Monoester Hydrolases/immunology,metabolism Phosphorylation tau Proteins/immunology
Chemicals
Antibodies, Monoclonal Epitopes Neurofilament Proteins tau Proteins Phosphoric Monoester Hydrolases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Mercken M
Innogenetics, Gent, Belgium.
Vandermeeren M
Lübke U
Six J
Boons J
Van de Voorde A
Martin J J
Gheuens J
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Article Info
Journal
Acta neuropathologica
Abbr.
Acta Neuropathol
ISSN
0001-6322
DOI
10.1007/BF00227819
Published
1992-00-00
Pages
265-72
Language
English
Region
Germany
NLM ID
0412041
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00227819 View journal details
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