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PMID: 1373018 Published · ppublish English Journal Article

Specific monoclonal antibodies against normal microtubule-associated protein-2 (MAP2) epitopes present in Alzheimer pathological structures do not recognize paired helical filaments.

Acta neuropathologica ·Vol. 83 ·No. 2 ·1992-00-00 ·Pages 179-89

Six J, Lübke U, Mercken M, Vandermeeren M, Ceuterick C, Van de Voorde A, Boons J, Gheuens J

Abstract

We have developed monoclonal antibodies that detect normal microtubule-associated protein-2 (MAP2) epitopes in routinely fixed, paraffin-embedded tissue. The somatodendritic distribution of MAP2 in bovine and human nervous tissue was confirmed with several of these antibodies. Furthermore, some of these antibodies immunohistochemically labeled certain pathological structures in Alzheimer brain, especially neurites in senile plaques. Electron microscopic observations, however, indicate that these MAP2 epitopes are not located in the Alzheimer paired helical filaments themselves, but in amorphous granular structures coexistent with them. While the pathological nature of these structures is undetermined, they may represent artefactual modifications of normal cytoskeletal components.

MeSH Terms
Alzheimer Disease/immunology,pathology Animals Antibodies, Monoclonal/immunology Cattle Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitopes/immunology Humans Hybridomas/immunology,metabolism Intermediate Filaments/immunology Mice Microscopy, Immunoelectron Microtubule-Associated Proteins/immunology Paraffin Embedding
Chemicals
Antibodies, Monoclonal Epitopes Microtubule-Associated Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Six J
Innogenetics, Ghent, Belgium.
Lübke U
Mercken M
Vandermeeren M
Ceuterick C
Van de Voorde A
Boons J
Gheuens J
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Article Info
Journal
Acta neuropathologica
Abbr.
Acta Neuropathol
ISSN
0001-6322
DOI
10.1007/BF00308477
Published
1992-00-00
Pages
179-89
Language
English
Region
Germany
NLM ID
0412041
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00308477 View journal details
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