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PMID: 1380697 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.

Nucleic acids research ·Vol. 20 ·No. 16 ·1992-08-25 ·Pages 4221-7

O'Connor M, Göringer HU, Dahlberg AE

Abstract

A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and -1 frameshifting in vivo. By contrast, mutations at position 534 had little or no effect on growth rate or translational fidelity. The results demonstrate the importance of G517 in maintaining translational fidelity but do not support a base pairing interaction between G517 and U534.

MeSH Terms
Base Sequence DNA Mutational Analysis Escherichia coli/genetics Molecular Sequence Data Mutation/genetics Nucleic Acid Conformation Plasmids/genetics Protein Biosynthesis/genetics RNA, Bacterial/genetics,metabolism RNA, Ribosomal, 16S/genetics,metabolism Recombinant Proteins/genetics
Chemicals
RNA, Bacterial RNA, Ribosomal, 16S Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
O'Connor M
Section of Biochemistry, Brown University, Providence, RI 02912.
Göringer H U
Dahlberg A E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.16.4221
Published
1992-08-25
Pages
4221-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334129
Subset
IM
Grants
NIGMS NIH HHS · GM19756 · United States
External Links
PubMed source DOI: 10.1093/nar/20.16.4221 PMC Full Text View journal details
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