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PMID: 1379716 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 6711-5

Lowenstein CJ, Glatt CS, Bredt DS, Snyder SH

Abstract

Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.

MeSH Terms
Amino Acid Oxidoreductases/genetics,metabolism Amino Acid Sequence Animals Base Sequence Brain/enzymology Cell Line Cloning, Molecular Humans Isoenzymes/genetics,metabolism Kinetics Macrophages/enzymology Mice Mice, Inbred BALB C Molecular Sequence Data Neurons/enzymology Nitric Oxide Synthase Oligonucleotides, Antisense Polymerase Chain Reaction RNA, Messenger/biosynthesis,genetics Recombinant Proteins/metabolism Sequence Homology, Nucleic Acid Spleen/enzymology Transfection
Chemicals
Isoenzymes Oligonucleotides, Antisense RNA, Messenger Recombinant Proteins Nitric Oxide Synthase Amino Acid Oxidoreductases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lowenstein C J
Department of Neuroscience, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
Glatt C S
Bredt D S
Snyder S H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.6711
Published
1992-08-01
Pages
6711-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49573
Subset
IM
Grants
NIGMS NIH HHS · GM-07309 · United States
NHLBI NIH HHS · HL-02451 · United States
NIMH NIH HHS · MH-18501 · United States
Databases
GENBANK
M92649
External Links
PubMed source DOI: 10.1073/pnas.89.15.6711 PMC Full Text View journal details
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