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PMID: 1376925 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 12 ·1992-06-15 ·Pages 5601-5

Bertran J, Werner A, Moore ML, Stange G, Markovich D, Biber J, Testar X, Zorzano A, Palacin M, Murer H

Abstract

We have isolated a cDNA clone by screening a rabbit kidney cortex cDNA library for expression of sodium-independent transport of L-arginine and L-alanine in Xenopus laevis oocytes. Expressed uptake relates to a single component of sodium-independent transport for dibasic and neutral amino acids. This transport activity resembles the functionally defined system b0,+ and carries cystine and dibasic amino acids with high affinity. The rBAT (b0,+ amino acid transporter-related) mRNA is found mainly in kidney and intestinal mucosa. It encodes a predicted 77.8-kDa protein with only one putative transmembrane domain and seven potential N-glycosylation sites. This protein could either be a constitutive element or a specific activator of system b0,+.

MeSH Terms
Amino Acid Sequence Amino Acids/metabolism Amino Acids, Diamino/metabolism Aminoisobutyric Acids/metabolism Animals Base Sequence Biological Transport Blotting, Northern Carrier Proteins/genetics,metabolism Cloning, Molecular Cystine/metabolism DNA/genetics,isolation & purification,metabolism Kidney Cortex/metabolism,physiology Membrane Proteins/genetics,metabolism Molecular Sequence Data Oocytes/metabolism,physiology RNA/genetics,isolation & purification Rabbits Rats Xenopus laevis
Chemicals
Amino Acids Amino Acids, Diamino Aminoisobutyric Acids Carrier Proteins Membrane Proteins 2-aminoisobutyric acid Cystine RNA DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Bertran J
Institute of Physiology, University of Zürich, Switzerland.
Werner A
Moore M L
Stange G
Markovich D
Biber J
Testar X
Zorzano A
Palacin M
Murer H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.12.5601
Published
1992-06-15
Pages
5601-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49340
Subset
IM
Databases
GENBANK
M90096
External Links
PubMed source DOI: 10.1073/pnas.89.12.5601 PMC Full Text View journal details
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