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PMID: 1374416 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of alpha 2 beta 1-mediated fibroblast migration on type I collagen by shifts in the concentrations of extracellular Mg2+ and Ca2+.

The Journal of cell biology ·Vol. 117 ·No. 5 ·1992-06-00 ·Pages 1109-17

Grzesiak JJ, Davis GE, Kirchhofer D, Pierschbacher MD

Abstract

Extracellular Ca2+ can reverse the Mg(2+)-dependent, alpha 2 beta 1-mediated adhesion of WI38 human fibroblasts to type I collagen substrates. Affinity chromatography data also demonstrate that Ca2+ can specifically elute the fibroblast alpha 2 beta 1 integrin bound to type I collagen-Sepharose in Mg2+. In modified Boyden chamber migration assays, Mg2+ alone supports the alpha 2 beta 1-mediated migration of fibroblasts on type I collagen substrates, while Ca2+ does not. However, a twofold enhancement in migration was observed when combinations of the two cations were used, with optimal migration observed when the Mg2+/Ca2+ ratio was higher than one. Inhibitory mAbs directed against various integrin subunits demonstrate that these observed cation effects appear to be mediated primarily by alpha 2 beta 1. These data, together with reports that under certain physiological conditions significant fluctuations in the concentrations of extracellular Ca2+ and Mg2+ can take place in vivo, suggest that the ratio between these two cations is involved in the up- and downregulation of integrin function, and thus, may influence cell migratory behavior.

MeSH Terms
Amino Acid Sequence Calcium/pharmacology Cell Movement/drug effects Chromatography, Affinity Collagen/metabolism Extracellular Matrix Proteins/metabolism Fibroblasts Glycoproteins/metabolism Humans Integrins/immunology,metabolism Magnesium/pharmacology Molecular Sequence Data Precipitin Tests Vitronectin
Chemicals
Extracellular Matrix Proteins Glycoproteins Integrins Vitronectin Collagen Magnesium Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grzesiak J J
La Jolla Cancer Research Foundation, California 92037.
Davis G E
Kirchhofer D
Pierschbacher M D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.117.5.1109
Published
1992-06-00
Pages
1109-17
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289472
Subset
IM
Grants
NCI NIH HHS · CA30199 · United States
NHLBI NIH HHS · HL38417 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.117.5.1109 PMC Full Text View journal details
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