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PMID: 2787511 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An alternative cytoplasmic domain of the integrin beta 3 subunit.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 14 ·1989-07-00 ·Pages 5415-8

van Kuppevelt TH, Languino LR, Gailit JO, Suzuki S, Ruoslahti E

Abstract

A cDNA encoding a new form of the shared beta subunit (beta 3) of the platelet integrin gpIIb/IIIa and the vitronectin receptor was isolated from a placental cDNA library by screening with a beta 3 (gpIIIa) DNA probe. This beta 3 variant differs from the previously reported beta 3 in that the cytoplasmic domain is 8 amino acids shorter and has an alternative, 13-amino acid COOH-terminal peptide. The 3' untranslated region of the cDNA also differs from the previously reported sequence, while the region coding for the transmembrane domain and extracellular domain is identical to it. Reverse transcription combined with polymerase chain reaction was used to show that human placental tissue and two human cell lines contain the variant mRNA. The sequences of the cDNAs for the previously known beta 3 and the variant beta 3 described here suggest that the difference between the cytoplasmic domains of these subunits arises as a result of an alternative mRNA splicing. These cytoplasmic domains may provide alternative means for the beta 3 integrins to interact with cytoskeletal components.

MeSH Terms
Amino Acid Sequence Antigens, Surface/genetics Base Sequence Cell Line Cloning, Molecular Cytoplasm/metabolism DNA/genetics Gene Amplification Genes Humans Integrins Macromolecular Substances Membrane Glycoproteins/genetics Molecular Sequence Data Oligonucleotide Probes Osteosarcoma
Chemicals
Antigens, Surface Integrins Macromolecular Substances Membrane Glycoproteins Oligonucleotide Probes DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van Kuppevelt T H
Cancer Research Center, La Jolla Cancer Research Foundation, CA 92037.
Languino L R
Gailit J O
Suzuki S
Ruoslahti E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.14.5415
Published
1989-07-00
Pages
5415-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297633
Subset
IM
Grants
NCI NIH HHS · CA 28896 · United States
NCI NIH HHS · CA 30199 · United States
NCI NIH HHS · CA 42507 · United States
Databases
GENBANK
M25108
External Links
PubMed source DOI: 10.1073/pnas.86.14.5415 PMC Full Text View journal details
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