Home LiteratureArticle Details
PMID: 1374415 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Requirement of the integrin beta 3 subunit for carcinoma cell spreading or migration on vitronectin and fibrinogen.

The Journal of cell biology ·Vol. 117 ·No. 5 ·1992-06-00 ·Pages 1101-7

Leavesley DI, Ferguson GD, Wayner EA, Cheresh DA

Abstract

FG human pancreatic carcinoma cells use integrin alpha v beta 5 as their primary vitronectin receptor since they fail to express integrin alpha v beta 3. These cells are unable to form focal contacts, spread, or migrate on vitronectin but readily do so on collagen in a beta 1 integrin-dependent manner. Transfection of FG cells with a cDNA encoding the integrin beta 3 subunit results in the surface expression of a functional integrin alpha v beta 3 heterodimer providing these cells with novel adhesive and biological properties. Specifically, FG cells expressing beta 3 acquire the capacity to attach and spread on vitronectin as well as fibrinogen with beta 3 localization to focal contacts. Moreover, these cells gain the capacity to migrate through a porous membrane in response to either vitronectin or fibrinogen. These results demonstrate that the beta 3 and beta 5 integrin subunits when associated with alpha v, promote distinct cellular responses to a vitronectin extracellular environment.

MeSH Terms
Cell Adhesion/physiology Cell Movement/physiology Fibronectins/metabolism Fluorescent Antibody Technique Glycoproteins/metabolism Humans Integrins/genetics,metabolism Transfection/genetics Tumor Cells, Cultured Vitronectin
Chemicals
Fibronectins Glycoproteins Integrins Vitronectin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Leavesley D I
Scripps Research Institute, La Jolla, California 92037.
Ferguson G D
Wayner E A
Cheresh D A
References (34)
34 references, click to expand
  1. Integrin, a transmembrane glycoprotein complex mediating cell-substratum adhesion.
    J Cell Sci Suppl. 1987;8:231-50 PMID: 3332662
  2. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  3. Complete amino acid sequence of a novel integrin beta subunit (beta 6) identified in epithelial cells using the polymerase chain reaction.
    J Biol Chem. 1990 Jul 15;265(20):11502-7 PMID: 2365683
  4. Platelet glycoprotein IIb-IIIa-like proteins mediate endothelial cell attachment to adhesive proteins and the extracellular matrix.
    J Biol Chem. 1987 Jul 25;262(21):9935-8 PMID: 2440865
  5. Biosynthetic and functional properties of an Arg-Gly-Asp-directed receptor involved in human melanoma cell attachment to vitronectin, fibrinogen, and von Willebrand factor.
    J Biol Chem. 1987 Dec 25;262(36):17703-11 PMID: 2447074
  6. Novel purification of vitronectin from human plasma by heparin affinity chromatography.
    Cell Struct Funct. 1988 Aug;13(4):281-92 PMID: 2460263
  7. Mechanism of fibronectin-mediated cell migration: dependence or independence of cell migration susceptibility on RGDS-directed receptor (integrin).
    Exp Cell Res. 1989 Jul;183(1):126-39 PMID: 2544438
  8. New perspectives in cell adhesion: RGD and integrins.
    Science. 1987 Oct 23;238(4826):491-7 PMID: 2821619
  9. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
    Annu Rev Cell Biol. 1988;4:487-525 PMID: 3058164
  10. Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin.
    Cell. 1986 Jul 18;46(2):271-82 PMID: 3487386
  11. Distinct biological consequences of integrin alpha v beta 3-mediated melanoma cell adhesion to fibrinogen and its plasmic fragments.
    J Biol Chem. 1992 Mar 15;267(8):5070-7 PMID: 1531979
  12. Characterization of the integrin alpha v beta 6 as a fibronectin-binding protein.
    J Biol Chem. 1992 Mar 25;267(9):5790-6 PMID: 1532572
  13. The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line.
    J Biol Chem. 1990 Apr 15;265(11):5938-41 PMID: 1690718
  14. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells.
    J Cell Biol. 1990 Apr;110(4):1387-404 PMID: 1691191
  15. Human microvascular endothelial cells use beta 1 and beta 3 integrin receptor complexes to attach to laminin.
    J Cell Biol. 1990 Sep;111(3):1233-43 PMID: 1697296
  16. Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
    J Cell Biol. 1991 May;113(4):919-29 PMID: 1709170
  17. Individual embryonic fibroblasts express multiple beta chains in association with the alpha v integrin subunit. Loss of beta 3 expression with cell confluence.
    J Biol Chem. 1991 Oct 5;266(28):18593-9 PMID: 1717451
  18. Cloning and expression of a divergent integrin subunit beta 8.
    J Biol Chem. 1991 Oct 15;266(29):19650-8 PMID: 1918072
  19. Expression of beta 1, beta 3, beta 4, and beta 5 integrins by human epidermal keratinocytes and non-differentiating keratinocytes.
    J Cell Biol. 1991 Nov;115(3):829-41 PMID: 1918165
  20. Patterning of the membrane cytoskeleton by the extracellular matrix.
    Semin Cell Biol. 1990 Oct;1(5):391-9 PMID: 1966329
  21. An interaction between alpha-actinin and the beta 1 integrin subunit in vitro.
    J Cell Biol. 1990 Aug;111(2):721-9 PMID: 2116421
  22. A novel fibronectin receptor with an unexpected subunit composition (alpha v beta 1).
    J Biol Chem. 1990 Apr 15;265(11):5934-7 PMID: 2138612
  23. Integrin distribution in malignant melanoma: association of the beta 3 subunit with tumor progression.
    Cancer Res. 1990 Oct 15;50(20):6757-64 PMID: 2208139
  24. cDNA sequence of the human integrin beta 5 subunit.
    J Biol Chem. 1990 Oct 5;265(28):17126-31 PMID: 2211615
  25. Cloning, primary structure and properties of a novel human integrin beta subunit.
    EMBO J. 1990 May;9(5):1561-8 PMID: 2328726
  26. Cloning of an integrin beta subunit exhibiting high homology with integrin beta 3 subunit.
    Proc Natl Acad Sci U S A. 1990 Jul;87(14):5354-8 PMID: 2371275
  27. Arg-Gly-Asp recognition by a cell adhesion receptor requires its 130-kDa alpha subunit.
    J Biol Chem. 1987 Feb 5;262(4):1434-7 PMID: 2433281
  28. Human endothelial cells synthesize and express an Arg-Gly-Asp-directed adhesion receptor involved in attachment to fibrinogen and von Willebrand factor.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6471-5 PMID: 2442758
  29. Fibronectin and vitronectin regulate the organization of their respective Arg-Gly-Asp adhesion receptors in cultured human endothelial cells.
    J Cell Biol. 1988 Sep;107(3):1215-23 PMID: 2458362
  30. A novel vitronectin receptor integrin (alpha v beta x) is responsible for distinct adhesive properties of carcinoma cells.
    Cell. 1989 Apr 7;57(1):59-69 PMID: 2467745
  31. A novel integrin beta subunit is associated with the vitronectin receptor alpha subunit (alpha v) in a human osteosarcoma cell line and is a substrate for protein kinase C.
    EMBO J. 1989 Oct;8(10):2955-65 PMID: 2479539
  32. Recognition of distinct adhesive sites on fibrinogen by related integrins on platelets and endothelial cells.
    Cell. 1989 Sep 8;58(5):945-53 PMID: 2673537
  33. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage.
    Nature. 1986 Apr 10-16;320(6062):531-3 PMID: 2938015
  34. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.117.5.1101
Published
1992-06-00
Pages
1101-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289489
Subset
IM
Grants
NCI NIH HHS · CA45726 · United States
NCI NIH HHS · CA50286 · United States
External Links
PubMed source DOI: 10.1083/jcb.117.5.1101 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2