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PMID: 1374057 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Membrane attack complex (MAC)-mediated damage to spermatozoa: protection of the cells by the presence on their membranes of MAC inhibitory proteins.

Immunology ·Vol. 75 ·No. 3 ·1992-03-00 ·Pages 499-506

Rooney IA, Davies A, Morgan BP

Abstract

Although antibody and complement are known to cause immobilization and killing of spermatozoa in vitro the components of the complement system mediating these effects remain undefined. Here we have examined the effects of the membrane attack complex (MAC) on spermatozoa and demonstrate that spermatotoxic effects are dependent on assembly of the complete MAC. We subsequently examined the presence and functional significance of the complement regulatory proteins decay accelerating factor (DAF), MAC-inhibiting protein (MIP) and CD59 antigen on spermatozoa. Both DAF and CD59 antigen were present on the membranes of these cells. Neutralization of CD59 antigen with specific antibodies increased the susceptibility of the cells to MAC-mediated damage, suggesting a role for this molecule in the protection of spermatozoa from complement-mediated damage in the female reproductive tract.

MeSH Terms
Antigens, CD/analysis CD55 Antigens CD59 Antigens Cell Survival/immunology Complement Membrane Attack Complex/antagonists & inhibitors,immunology Flow Cytometry Fluorescent Antibody Technique Humans Infertility, Male/immunology Male Membrane Glycoproteins/analysis Membrane Proteins/analysis Proteins/analysis Sperm Motility/physiology Spermatozoa/immunology
Chemicals
Antigens, CD CD55 Antigens CD59 Antigens Complement Membrane Attack Complex Membrane Glycoproteins Membrane Proteins Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rooney I A
Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, U.K.
Davies A
Morgan B P
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1992-03-00
Pages
499-506
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1384746
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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