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PMID: 1372592 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression and function of membrane attack complex inhibitory proteins on thyroid follicular cells.

Immunology ·Vol. 75 ·No. 2 ·1992-02-00 ·Pages 372-7

Tandon N, Morgan BP, Weetman AP

Abstract

Human thyroid cells are resistant to lysis by the homologous membrane attack complex. By immunohistochemical staining we here show that normal thyroid cells and those in Graves' disease and Hashimoto's thyroiditis express two membrane attack complex-inhibiting proteins, CD59 antigen and membrane attack complex-inhibiting protein/homologous restriction factor (MIP/HRF). In vitro, the expression of both molecules was enhanced by interleukin-1 (IL-1), tumour necrosis factor (TNF) and interferon-gamma (IFN-gamma) and cytokine-treated thyroid cells were more resistant to lysis by homologous complement. Blocking experiments with monoclonal antibodies against CD59 antigen and MIP/HRF showed that both molecules contributed but CD59 antigen was the more important in mediating resistance to complement attack. Expression of these proteins may be an important determinant of the severity of tissue injury produced by complement-fixing thyroid peroxidase antibodies in autoimmune thyroid disease.

MeSH Terms
Antigens, CD/analysis Blood Proteins/analysis Blotting, Western CD59 Antigens Carrier Proteins/analysis Cell Membrane/immunology Cell Survival/immunology Cells, Cultured Complement Membrane Attack Complex/antagonists & inhibitors Graves Disease/immunology Humans Membrane Glycoproteins/analysis Proteins/analysis Thyroid Gland/immunology Thyroiditis, Autoimmune/immunology
Chemicals
Antigens, CD Blood Proteins CD59 Antigens Carrier Proteins Complement Membrane Attack Complex Membrane Glycoproteins Proteins CD59 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tandon N
Department of Medicine, University of Sheffield Clinical Sciences Centre, Northern General Hospital, U.K.
Morgan B P
Weetman A P
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28 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1992-02-00
Pages
372-7
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1384722
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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