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PMID: 1372316 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Construction of Salmonella strains with both antigen O4 (of group B) and antigen O9 (of group D).

Journal of bacteriology ·Vol. 174 ·No. 6 ·1992-03-00 ·Pages 1911-5

Johnson BN, Weintraub A, Lindberg AA, Stocker BA

Abstract

A Salmonella live vaccine causing both O4- and O9-specific immune responses would be of use, but no reported Salmonella serotype has both of these O antigens. Constructed Salmonella typhimurium strains with an rfb (O-antigen-specifying) gene cluster of type D in the chromosome and one of type B in an F'-rfb+ factor, and those with the reverse combination reacted strongly with both anti-O4 (and anti-O5) and anti-O9 sera and, if they carried recA, could be maintained in this state by growth conditions selective for retention of the F' factor. One of the two B.rfb+ gene clusters of a (P22-lysogenic) S. typhimurium strain with a tandem duplication of a chromosomal segment including hisD and B.rfb+ was replaced (by transduction) by a D.rfb+ gene cluster; the resulting strain was O1+ O4+ O5+ O9+ and stable as such after being made recA. A stable O4+ O9+ derivative of a virulent S. enteritidis (O-group D) strain was made by transducing into it first the join point of an appropriate tandem duplication strain, together with the adjacent B.rfb+ gene cluster, and then srl::Tn10 recA.

Related Genes
rfb
MeSH Terms
Cloning, Molecular F Factor Genes, Bacterial Lipopolysaccharides/chemistry O Antigens Polysaccharides, Bacterial/genetics,immunology Salmonella enteritidis/genetics,immunology Salmonella typhimurium/genetics,immunology
Chemicals
Lipopolysaccharides O Antigens Polysaccharides, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Johnson B N
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305.
Weintraub A
Lindberg A A
Stocker B A
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17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.6.1911-1915.1992
Published
1992-03-00
Pages
1911-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205796
Subset
IM
Grants
NIAID NIH HHS · AI10768 · United States
NIAID NIH HHS · AI18872 · United States
External Links
PubMed source DOI: 10.1128/jb.174.6.1911-1915.1992 PMC Full Text View journal details
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