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PMID: 1372164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of the complement-inhibiting protein CD59 antigen from platelet membranes.

The Biochemical journal ·Vol. 282 ( Pt 2) ·1992-03-01 ·Pages 409-13

Morgan BP

Abstract

Several groups have recently described the isolation of a 20 kDa membrane-attack-complex (MAC)-inhibiting protein, termed 'CD59 antigen', from human erythrocyte membranes. Antibodies raised against erythrocyte CD59 antigen detect antigen on the surface of many other cell types, and in some of these cells the antigen has been shown to have a molecular mass similar to that of the erythrocyte protein and to confer resistance to lysis by the MAC. A platelet-membrane form of CD59 antigen has been described and reported to be much larger than the erythrocyte protein. Here I report the isolation of CD59 antigen from platelet membranes and its molecular and functional characterization. The platelet protein is not significantly larger than the erythrocyte form and possesses similar MAC-inhibiting activity. Platelet CD59 antigen is anchored to the membrane via a glycosyl-phosphatidylinositol link, and consequently it is suggested that deficiency of this protein might be responsible for the increased thrombotic tendency observed in paroxysmal nocturnal haemoglobinuria.

MeSH Terms
Antigens, CD/isolation & purification,metabolism Blood Platelets/metabolism Blotting, Western CD59 Antigens Cell Membrane/metabolism Electrophoresis, Polyacrylamide Gel Flow Cytometry Humans Membrane Glycoproteins/isolation & purification,metabolism Phosphatidylinositol Diacylglycerol-Lyase Phosphoric Diester Hydrolases/metabolism
Chemicals
Antigens, CD CD59 Antigens Membrane Glycoproteins Phosphoric Diester Hydrolases Phosphatidylinositol Diacylglycerol-Lyase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Morgan B P
Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, U.K.
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27 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2820409
Published
1992-03-01
Pages
409-13
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1130793
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1042/bj2820409 PMC Full Text View journal details
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