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PMID: 13706206 Published · ppublish English Journal Article

A correlative study by electron and light microscopy of the development of type 5 adenovirus. II. Light microscopy.

The Journal of experimental medicine ·Vol. 112 ·1960-08-01 ·Pages 383-402

GODMAN GC, MORGAN C, BREITENFELD PM, ROSE HM

Abstract

The evolution of the intranuclear lesion produced by type 5 adenovirus in HEp-2 and HeLa cells is described as seen in the light microscope and the bodies formed in the course of the infection characterized histochemically. Some 12 hours after infection acidophilic protein bodies, without appreciable nucleic acid, first appear in the nucleus and coalesce into a network. Within or in association with this material, DNA-containing masses (viral aggregates) are formed which rapidly increase in amount and then coalesce. At the same time, a protein is produced, histochemically different from that of the acidophilic or basophilic structures mentioned, within the infected nucleus, which constitutes a matrix within which regular cytstals of a protein, (presumably non-viral) materialize. These structural and histochemical features are correlated with details which have been observed in parallel studies with the electron microscope.

Keywords
ADENOVIRUS
MeSH Terms
Adenoviridae Cell Nucleus DNA Electrons HeLa Cells Humans Microscopy
Chemicals
DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
GODMAN G C
MORGAN C
BREITENFELD P M
ROSE H M
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40 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.112.2.383
Published
1960-08-01
Pages
383-402
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2137218
Subset
OM
External Links
PubMed source DOI: 10.1084/jem.112.2.383 PMC Full Text View journal details
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