Intracellular localization of type 4 adenovirus. II. Cytological and fluorescein-labelled antibody studies.
HeLa cell cultures infected with adenovirus type 4 were studied by light and phase-contrast microscopy and by the fluorescent antibody technique for visualization of intracellular antigen. The findings were correlated with the growth curve of infectious virus, determined from companion cultures. The results indicated that those cells undergoing characteristic structural changes observable by light microscopy were those which contain viral antigen. The distribution of the majority of the antigen within the infected cells corresponded to that of the regularly aligned granules and crystal-like masses seen in the nuclei of cells in stained and in unfixed cultures. The production of infectious virus was closely correlated with the development of the characteristic nuclear changes.
16 references, click to expand
-
Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.J Exp Med. 1950 Jan 1;91(1):1-13 PMID: 15395569
-
Electron microscopy of HeLa cells infected with adenoviruses.J Exp Med. 1956 Sep 1;104(3):443-54 PMID: 13357696
-
Crystalline-like patterns in the nuclei of cells infected with an animal virus.Nature. 1955 Mar 19;175(4455):505-6 PMID: 14370153
-
The occurrence of intranuclear crystals in living HeLa cells infected with adenoviruses.J Biophys Biochem Cytol. 1957 Mar 25;3(2):323-4 PMID: 13438915
-
Cellular localization of Herpes simplex virus by means of fluorescent antibody.Virology. 1956 Aug;2(4):496-510 PMID: 13352775
-
Characteristics of the new respiratory viruses (adenoviruses). I. Qualitative and quantitative aspects of the neutralization reaction.J Immunol. 1956 Oct;77(4):271-8 PMID: 13376899
-
Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix.J Exp Med. 1953 May;97(5):695-710 PMID: 13052828
-
Etiologic relationship of the RI-67 agent to acute respiratory disease (ARD).J Clin Invest. 1955 Jun;34(6):820-31 PMID: 14381511
-
Cytological and cytochemical studies of HeLa cells infected with adeno-viruses.J Exp Med. 1957 Mar 1;105(3):195-216 PMID: 13406179
-
Intracellular localization of type 4 adenovirus. I. Cellular fractionation studies.J Exp Med. 1959 Jan 1;109(1):69-83 PMID: 13611165
-
The growth curve of the virus of herpes simplex in rabbit corneal cells grown in tissue culture with parallel observations on the development of the intranuclear inclusion body.J Immunol. 1953 Dec;71(6):385-96 PMID: 13118177
-
Tryptose phosphate broth as supplementary factor for maintenance of HeLa cell tissue cultures.Proc Soc Exp Biol Med. 1955 May;89(1):66-71 PMID: 14384881
-
The packing structure of crystalline RI-APC virus.J Biophys Biochem Cytol. 1956 Jul 25;2(4):483-6 PMID: 13357513
-
Recovery of new agent from patients with acute respiratory illness.Proc Soc Exp Biol Med. 1954 Jan;85(1):183-8 PMID: 13134329
-
Cytochemical studies on the intranuclear inclusion of herpes simplex.J Immunol. 1950 Jul;65(1):119-28 PMID: 15436991
-
Dose-response curves of toxic and infective actions of adenovirus in HeLa cell cultures.J Gen Microbiol. 1957 Oct;17(2):517-24 PMID: 13481333
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