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PMID: 1369160 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of Streptomyces genes encoding extracellular enzymes in Brevibacterium lactofermentum: secretion proceeds by removal of the same leader peptide as in Streptomyces lividans.

Applied microbiology and biotechnology ·Vol. 38 ·No. 3 ·1992-12-00 ·Pages 362-9

Cadenas RF, Gil JA, Martín JF

Abstract

The alpha-amylase gene (amy) from Streptomyces griseus IMRU 3570 and the beta-galactosidase gene (lac) from S. lividans were subcloned into Brevibacterium lactofermentum or B. lactofermentum/Escherichia coli shuttle vectors. The amy gene was not expressed in B. lactofermentum from its own promoter but was efficiently expressed when the promoter of the kanamycin resistance gene (kan) was inserted upstream of the promoterless amylase gene. The lac gene from S. lividans was subcloned without its native promoter and was expressed when placed downstream of pBL1 promoters P2 or P3. The alpha-amylase was secreted extracellularly by removal of the same 28-amino acid leader peptide as in S. lividans. The amy and lac genes provide useful markers for selection of transformants and will facilitate the study of protein secretion in B. lactofermentum.

Related Genes

amy kan lac

MeSH Terms

Amino Acid Sequence Biotechnology Brevibacterium/genetics Cloning, Molecular Gene Expression Genes, Bacterial Genetic Vectors Kanamycin Resistance/genetics Lac Operon Molecular Sequence Data Plasmids Protein Sorting Signals/genetics Streptomyces/enzymology,genetics Streptomyces griseus/enzymology,genetics alpha-Amylases/genetics beta-Galactosidase/genetics

Chemicals

Protein Sorting Signals alpha-Amylases beta-Galactosidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cadenas R F

Area de Microbiología, Facultad de Biología, Universidad de León, Spain.

Gil J A

Martín J F

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Article Info

Journal

Applied microbiology and biotechnology

Abbr.

Appl Microbiol Biotechnol

ISSN

0175-7598

DOI

10.1007/BF00170087

Published

1992-12-00

Pages

362-9

Language

English

Region

Germany

NLM ID

8406612

External Links

PubMed source DOI: 10.1007/BF00170087 View journal details

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Expression of Streptomyces genes encoding extracellular enzymes in Brevibacterium lactofermentum: secretion proceeds by removal of the same leader peptide as in Streptomyces lividans.

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