Characterization of the promoter, signal sequence, and amino terminus of a secreted beta-galactosidase from "Streptomyces lividans".

Journal of bacteriology ·Vol. 169 ·No. 9 ·1987-09-00 ·Pages 4249-56

Eckhardt T, Strickler J, Gorniak L, Burnett WV, Fare LR

Abstract

The gene for a secreted 130-kilodalton beta-galactosidase from "Streptomyces lividans" has been cloned, its promoter, signal sequence, and amino terminal region have been localized, and their nucleotide sequence has been determined. The signal sequence extends over 56 amino acids and shows the characteristic-features of signal sequences, including a hydrophilic amino terminus followed by a hydrophobic core near the signal cleavage site. The secretion of beta-galactosidase depends on the presence of the signal sequence. beta-Galactosidase is the major protein in culture supernatants and extracts of strains expressing the cloned beta-galactosidase gene and represents a valuable tool in the study of protein secretion in Streptomyces spp.

MeSH Terms

Base Sequence Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/analysis Deoxyribonuclease BamHI Endonucleases Galactosidases/genetics Genes, Bacterial Promoter Regions, Genetic Protein Biosynthesis Protein Sorting Signals/genetics RNA, Bacterial/analysis RNA, Messenger/genetics Single-Strand Specific DNA and RNA Endonucleases Streptomyces/enzymology,genetics beta-Galactosidase/genetics

Chemicals

DNA, Bacterial Protein Sorting Signals RNA, Bacterial RNA, Messenger Endonucleases DNA Restriction Enzymes Deoxyribonuclease BamHI Single-Strand Specific DNA and RNA Endonucleases Galactosidases beta-Galactosidase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Eckhardt T

Strickler J

Gorniak L

Burnett W V

Fare L R

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22 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.169.9.4249-4256.1987

Published

1987-09-00

Pages

4249-56

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC213737

Subset

Databases

GENBANK

M17359

External Links

PubMed source DOI: 10.1128/jb.169.9.4249-4256.1987 PMC Full Text View journal details

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Characterization of the promoter, signal sequence, and amino terminus of a secreted beta-galactosidase from "Streptomyces lividans".

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