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PMID: 1363436 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Calcium current modulation in frog sympathetic neurones: L-current is relatively insensitive to neurotransmitters.

The Journal of physiology ·Vol. 456 ·1992-10-00 ·Pages 107-23

Elmslie KS, Kammermeier PJ, Jones SW

Abstract

1. Neurotransmitters (noradrenaline, NA; chicken II luteinizing hormone-releasing hormone, LHRH) and activators of G proteins (GTP-gamma-S and AlF3) partially inhibit calcium current in bullfrog sympathetic neruones. Activation of the remaining current is slowed and shifted to more positive voltages. 2. The N-type calcium current appears to be the type modulated, since approximately 90% of peak current is blocked by omega-conotoxin (omega CgTx) and modulation is not affected by nisoldipine. 3. Calcium current at relatively negative voltages (-30 to -50 mV) is resistant to transmitter modulation. The current at such voltages is also resistant to omega CgTx, suggesting that it results from a different type of calcium channel. 4. The omega CgTx-resistant current includes dihydropyridine (DHP)-sensitive and DHP-resistant components. The omega CgTx- and DHP-resistant current is inhibited by transmitter agonist, but the DHP-sensitive (L-type) current is not. 5. In cells dialysed with a low concentration of calcium buffer (0.1 mM-BAPTA), transmitters still inhibit N-current incompletely. However, L-current was partially inhibited (approximately 10%) by LHRH, NA and the muscarinic agonist oxotremorine-M (OXO-M).

MeSH Terms
Aluminum/pharmacology Aluminum Compounds Animals Calcium Channels/drug effects Dose-Response Relationship, Drug Fluorides/pharmacology Ganglia, Sympathetic/physiology Gonadotropin-Releasing Hormone/pharmacology Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology In Vitro Techniques Neurotransmitter Agents/pharmacology Nifedipine/pharmacology Norepinephrine/pharmacology Peptides, Cyclic/pharmacology Rana catesbeiana omega-Conotoxin GVIA
Chemicals
Aluminum Compounds Calcium Channels Neurotransmitter Agents Peptides, Cyclic Gonadotropin-Releasing Hormone Guanosine 5'-O-(3-Thiotriphosphate) omega-Conotoxin GVIA Aluminum Nifedipine Fluorides Norepinephrine aluminum fluoride
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Elmslie K S
Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106.
Kammermeier P J
Jones S W
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34 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1992.sp019329
Published
1992-10-00
Pages
107-23
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1175674
Subset
IM
Grants
NINDS NIH HHS · NS 08528 · United States
NINDS NIH HHS · NS 24471 · United States
External Links
PubMed source DOI: 10.1113/jphysiol.1992.sp019329 PMC Full Text View journal details
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