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PMID: 133678 Published · ppublish English Comparative Study Journal Article

Phosphorylation state of cytosolic and mitochondrial adenine nucleotides and of pyruvate dehydrogenase in isolated rat liver cells.

The Biochemical journal ·Vol. 156 ·No. 1 ·1976-04-15 ·Pages 91-102

Siess EA, Wieland OH

Abstract

1. Cytosolic and mitochondrial ATP and ADP concentrations of liver cells isolated from normal fed, starved and diabetic rats were determined. 2. The cytosolic ATP/ADP ratio was 6,9 and 10 in normal fed, starved and diabetic rats respectively. 3. The mitochondrial ATP/ADP ratio was 2 in normal and diabetic rats and 1.6 in starved rats. 4. Adenosine increased the cytosolic and lowered the mitochondrial ATP/ADP ratio, whereas atractyloside had the opposite effect. 5. Incubation of the hepatocytes with fructose, glycerol or sorbitol led to a fall in the ATP/ADP ratio in both the cytosolic and the mitochondrial compartment. 6. The interrelationship between the mitochondrial ATP/ADP ratio and the phosphorylation state of pyruvate dehydrogenase in intact cells was studied. 7. In hepatocytes isolated from fed rats an inverse correlation between the mitochondrial ATP/ADP ratio and the active form of pyruvate dehydrogenase (pyruvate dehydrogenase a) was demonstrable on loading with fructose, glycerol or sorbitol. 8. No such correlation was obtained with pyruvate or dihydroxyacetone. For pyruvate, this can be explained by inhibition of pyruvate dehydrogenase kinase. 9. Liver cells isolated from fed animals displayed pyruvate dehydrogenase a activity twice that found in vivo. Physiological values were obtained when the hepatocytes were incubated with albumin-oleate, which also yielded the highest mitochondrial ATP/ADP ratio.

MeSH Terms
Acetoacetates/analysis Adenosine Diphosphate/metabolism Adenosine Triphosphate/metabolism Ammonium Chloride/pharmacology Animals Atractyloside/pharmacology Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Coenzyme A/analysis Cytosol/metabolism Diabetes Mellitus/chemically induced,metabolism Diabetes Mellitus, Experimental/metabolism Hydroxybutyrates/analysis In Vitro Techniques Liver/metabolism Male Mitochondria, Liver/metabolism Pyruvate Dehydrogenase Complex/metabolism Rats Starvation Streptozocin
Chemicals
Acetoacetates Hydroxybutyrates Pyruvate Dehydrogenase Complex Ammonium Chloride Atractyloside Carbonyl Cyanide m-Chlorophenyl Hydrazone Streptozocin Adenosine Diphosphate Adenosine Triphosphate Coenzyme A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Siess E A
Wieland O H
References (51)
51 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1560091
Published
1976-04-15
Pages
91-102
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1163720
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1560091 PMC Full Text View journal details
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