Measurement of the ATP/ADP ratio in mitochondria and in the extramitochondrial compartment by fractionation of freeze-stopped liver tissue in non-aqueous media.

Acid Phosphatase/metabolism Adenine Nucleotides/analysis Adenosine Diphosphate/analysis Adenosine Triphosphate/analysis Animals Cell Fractionation Centrifugation, Density Gradient Citrate (si)-Synthase/metabolism Citrates/metabolism Culture Media Cytochrome Reductases/metabolism Fluorometry Freezing Glutamate Dehydrogenase/metabolism In Vitro Techniques L-Lactate Dehydrogenase/metabolism Liver/metabolism,ultrastructure Lysosomes/enzymology Malates/metabolism Male Microscopy, Electron Mitochondria, Liver/analysis,enzymology NADP Nucleotidyltransferases/metabolism Oxygen Consumption Perfusion Phosphoglycerate Kinase/metabolism Proteins/analysis Rats

Adenine Nucleotides Citrates Culture Media Malates Proteins NADP Adenosine Diphosphate Adenosine Triphosphate L-Lactate Dehydrogenase Glutamate Dehydrogenase Cytochrome Reductases Citrate (si)-Synthase Phosphoglycerate Kinase Nucleotidyltransferases Acid Phosphatase