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PMID: 1333610 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Positive supercoiling of DNA greatly diminishes mRNA synthesis in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 23 ·1992-12-01 ·Pages 11461-5

Gartenberg MR, Wang JC

Abstract

In Saccharomyces cerevisiae cells harboring a GAL1 promoter-linked beta-galactosidase gene, the simultaneous expression of Escherichia coli DNA topoisomerase I and inactivation of yeast DNA topoisomerases I and II reduces the cellular level of beta-galactosidase to an undetectable level. Analysis of intracellular mRNA level and the density of RNA polymerase along DNA indicates that this reduction is due to the suppression of transcription and that both plasmid-borne and chromosomally located genes are affected. These results are interpreted in terms of inhibition of transcription in vivo due to positive supercoiling of the DNA template: preferential removal of transcription-generated negative supercoils by E. coli DNA topoisomerase I in the absence of both yeast DNA topoisomerases I and II results in the accumulation of positive supercoils in intracellular DNA. In normal prokaryotic or eukaryotic cells, accumulation of positive supercoils is presumably avoided through the balanced actions of DNA topoisomerases.

Related Genes
ADH1 GAL1 URA3 top1 top2
MeSH Terms
Base Sequence DNA Topoisomerases, Type I/metabolism DNA, Fungal/genetics DNA, Superhelical/genetics Genes, Fungal Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides/chemistry Plasmids RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Templates, Genetic Transcription, Genetic
Chemicals
DNA, Fungal DNA, Superhelical Oligodeoxyribonucleotides RNA, Messenger DNA Topoisomerases, Type I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gartenberg M R
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Wang J C
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.23.11461
Published
1992-12-01
Pages
11461-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50571
Subset
IM
Grants
NIGMS NIH HHS · GM24544 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.23.11461 PMC Full Text View journal details
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